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Titolo:
DNA microarrays and beyond: completing the journey from tissue to cell
Autore:
Mills, JC; Roth, KA; Cagan, RL; Gordon, JI;
Indirizzi:
Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 & Pharmacol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 l & Immunol, St Louis, MO 63110 USA
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 8, volume: 3, anno: 2001,
pagine: E175 - E178
SICI:
1465-7392(200108)3:8<E175:DMABCT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LASER CAPTURE MICRODISSECTION; IN-SITU HYBRIDIZATION; TYRAMIDE SIGNAL AMPLIFICATION; GENE-EXPRESSION; OLIGONUCLEOTIDE ARRAYS; PCR; IDENTIFICATION;
Tipo documento:
Editorial Material
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Cagan, RL Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 l, St Louis, MO 63110 USA
Citazione:
J.C. Mills et al., "DNA microarrays and beyond: completing the journey from tissue to cell", NAT CELL BI, 3(8), 2001, pp. E175-E178

Abstract

For the cell biologist, identifying changes in gene expression using DNA microarrays is,just the start of along journey from tissue to cell. We discuss how chip users can first filter noise (false-positives) from daunting microarray datasets. Combining laser capture microdissection with real-time polymerase chain reaction and reverse transcription is a helpful follow-up step that allows expression of selected genes to be quantified in populations of recovered cells. The voyage from chip to single cell can be completed using sensitive new in situ hybridization and immunohistochemical methods based on tyramide signal amplification.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 23:00:06