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Titolo:
Production and neurotropism of lentivirus vectors pseudotyped with lyssavirus envelope glycoproteins
Autore:
Desmaris, N; Bosch, A; Salaun, C; Petit, C; Prevost, MC; Tordo, N; Perrin, P; Schwartz, O; de Rocquigny, H; Heard, JM;
Indirizzi:
Inst Pasteur, Unite Retrovirus & Transfert Genet, CNRS, URA 1930, F-75724 Paris, France Inst Pasteur Paris France F-75724 CNRS, URA 1930, F-75724 Paris, France Inst Pasteur, Lab Microscopie Elect, F-75724 Paris, France Inst Pasteur Paris France F-75724 croscopie Elect, F-75724 Paris, France Inst Pasteur, Lab Lyssavirus, F-75724 Paris, France Inst Pasteur Paris France F-75724 Lab Lyssavirus, F-75724 Paris, France
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 2, volume: 4, anno: 2001,
pagine: 149 - 156
SICI:
1525-0016(200108)4:2<149:PANOLV>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE LEUKEMIA-VIRUS; VESICULAR STOMATITIS-VIRUS; MUCOPOLYSACCHARIDOSIS TYPE-VII; RABIES VIRUS; GENE-TRANSFER; RETROVIRAL VECTORS; NONDIVIDING CELLS; NERVOUS-SYSTEM; HIGH-TITER; EFFICIENT;
Keywords:
lentivirus vector; rabies virus; Mokola; VSV-G; central nervous system; neuronal tropism; retrograde axonal transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Heard, JM Inst Pasteur, Unite Retrovirus & Transfert Genet, CNRS, URA 1930, 28 Rue Dr Roux, F-75724 Paris, France Inst Pasteur 28 Rue Dr Roux Paris France F-75724 Paris, France
Citazione:
N. Desmaris et al., "Production and neurotropism of lentivirus vectors pseudotyped with lyssavirus envelope glycoproteins", MOL THER, 4(2), 2001, pp. 149-156

Abstract

We investigated the production efficiency and the gene transfer capacity in the central nervous system of HIV-1-based vectors pseudotyped with eitherthe G protein of the Mokola lyssaviruses (MK-G), a neurotropic virus causing rabies disease, or the vesiculo-stomatitis G protein (VSV-G). Both envelopes induced syncitia in cell cultures. They were incorporated into vector particles and mature virions were observed by electron microscopy. Vector production was two- to sixfold more efficient with VSV-G than with MK-G. Forequivalent amounts of physical particles, vector titration was 5- to 25-fold higher with VSV-G than with MK-G pseudotypes on cultured cells, and in vivo gene expression in mouse brain was more intense. Thus, VSV-G pseudotypes were produced more efficiently and were more infectious than MK-G pseudotypes. Tropism for brain cells was analyzed by intrastriatal injections in rats. Both pseudotypes preferentially transduced neurons (70-90% of transduced cells). Retrograde axonal transport was investigated by instilling vector suspensions in the rat nasal cavity. Both pseudotypes were efficiently transported to olfactive neuron bodies. Thus, although coating HIV-1 particles with rabdhovirus envelope glycoproteins enables them to enter neuronal cells efficiently, pseudotyping is not sufficient to confer the powerful neurotropism of lyssaviruses to lentivirus vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:07:29