Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The ovulatory gonadotrophin surge stimulates cyclooxygenase expression andprostaglandin production by the monkey follicle
Autore:
Duffy, DM; Stouffer, RL;
Indirizzi:
Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR 97006 USA OregonReg Primate Res Ctr Beaverton OR USA 97006 Beaverton, OR 97006 USA Oregon Hlth Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 rmacol, Portland, OR 97201 USA
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 8, volume: 7, anno: 2001,
pagine: 731 - 739
SICI:
1360-9947(200108)7:8<731:TOGSSC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; BOVINE PREOVULATORY FOLLICLES; HUMAN CHORIONIC-GONADOTROPIN; HCG INDUCED OVULATION; RHESUS-MONKEY; ENDOPEROXIDE SYNTHASE-2; GRANULOSA-CELLS; MICE LACKING; IN-VITRO; RECEPTOR EXPRESSION;
Keywords:
cyclooxygenase; follicle; gonadotrophin surge; ovulation; prostaglandin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Duffy, DM Oregon Reg Primate Res Ctr, Div Reprod Sci, 505 NW 185th Ave, Beaverton, OR 97006 USA Oregon Reg Primate Res Ctr 505 NW 185th Ave BeavertonOR USA 97006
Citazione:
D.M. Duffy e R.L. Stouffer, "The ovulatory gonadotrophin surge stimulates cyclooxygenase expression andprostaglandin production by the monkey follicle", MOL HUM REP, 7(8), 2001, pp. 731-739

Abstract

Follicular prostaglandin concentrations increase following the gonadotrophin surge in domestic animals and rodents similar to 10 h before follicle rupture, suggesting a unifying role for prostaglandins in the timing of ovulation. However, little is known about prostaglandin production by the primate ovulatory follicle. In this study, adult female macaques received gonadotrophins to promote follicular development. Granulosa cells, follicular fluid, and ovaries were collected before (0 h) and 12, 24 or 36 h after administration of the ovulatory stimulus, human chorionic gonadotrophin (HCG). Cyclooxygenase (COX) isoform expression was assessed by reverse transcription-polymerase chain reaction and immunocytochemistry and follicular prostaglandin production was determined by enzyme immunoassay. COX-2 mRNA expression in granulosa cells was low at 0 h, rose 50-fold by 12 h, and remained elevated through to 36 h. COX-2 immunostaining was present in granulosa cells after, but not before, exposure to HCG. COX-1 mRNA levels did not change during the periovulatory interval, and COX-1 immunostaining of granulosa cells was not detected. Follicular fluid prostaglandin (PG) E-2 and PGF(2 alpha),concentrations were low through to 24 h but increased 100-fold at 36 h. The elevated follicular prostaglandin concentrations 4-16 h before the expected time of ovulation support the hypothesis that the time between the LH surge and increased follicular prostaglandins determines the length of the periovulatory period. Differences between the localization and timing of COX-2 expression in monkey versus non-primate follicles suggest that the pattern of COX-2 expression and activity has aspects unique to primates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 08:15:43