Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Protein tyrosine kinase expression in the porcine ovary
Autore:
Okamura, Y; Myoumoto, A; Manabe, N; Tanaka, N; Okamura, H; Fukumoto, M;
Indirizzi:
Tohoku Univ, Inst Dev Aging & Canc, Dept Pathol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan Kyoto Univ, Grad Sch Med, Dept Pathol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 pt Pathol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ, Grad Sch Agr, Dept Anim Sci, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Anim Sci, Sakyo Ku, Kyoto 6068501, Japan Kumamoto Univ, Sch Med, Dept Obstet & Gynecol, Kumamoto 8608556, Japan Kumamoto Univ Kumamoto Japan 8608556 & Gynecol, Kumamoto 8608556, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 8, volume: 7, anno: 2001,
pagine: 723 - 729
SICI:
1360-9947(200108)7:8<723:PTKEIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; GROWTH-FACTOR-I; RECEPTOR GENE-EXPRESSION; MACAQUE GRANULOSA-CELLS; FOCAL ADHESION KINASE; C-KIT RECEPTOR; DEVELOPMENTAL EXPRESSION; FOLLICULAR DEVELOPMENT; MOUSE DEVELOPMENT; CUMULUS CELLS;
Keywords:
folliculogenesis; granulosa cell; oocyte; porcine; tyrosine kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Fukumoto, M Tohoku Univ, Inst Dev Aging & Canc, Dept Pathol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Miyagi 9808575, Japan
Citazione:
Y. Okamura et al., "Protein tyrosine kinase expression in the porcine ovary", MOL HUM REP, 7(8), 2001, pp. 723-729

Abstract

Various growth factor receptors contain intrinsic tyrosine kinase activity, indicating that protein tyrosine kinases (PTK) play an important role in signal transduction pathways for cell proliferation and differentiation. Toidentify oocyte-derived factors which control follicle cells as well as oocyte-controlling factors produced by follicle cells, we examined the expression of genes which contain the PTK domain in the porcine ovary, using a polymerase chain reaction-based amplification technique with degenerate oligonucleotide primers that are specific to the PTK domain. Clones for the porcine homologues of platelet-derived growth factor receptor alpha (PDGFRalpha) and of insulin-like growth factor-I receptor (IGF-IR) were found during follicle growth both in oocytes and follicle cells. Clones for the porcine homologues of focal adhesion kinase (FAK), of c-kit and of fms-like tyrosinekinase (FLT)-3 were found only in oocytes. Moreover, after 24 h of in-vitro maturation of the cumulus-oocyte complexes, clones for the porcine homologues of FLT-1, of FLT-4, of Tie2 and of RYK in oocytes were observed. Immunohistochemical studies revealed the existence of PDGFR alpha, platelet-derived growth factor alpha (PDGFralpha), FAK and FLT3 in oocytes at various stages of folliculogenesis. These results suggest that fluctuations in the expression of these PTK genes may be involved in follicle growth and maturation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:29:18