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Titolo:
Transcription of mutS- and mutL-homologous genes during meiosis in Saccharomyces cerevisiae and identification of a regulatory cis-element for meiotic induction of MSH2
Autore:
Meyer, C; Scheller, J; Kramer, W;
Indirizzi:
Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Genet & Praparat Mol Biol,D-37077 Gottingen, Germany Univ Gottingen Gottingen Germany D-37077 Biol,D-37077 Gottingen, Germany
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 5, volume: 265, anno: 2001,
pagine: 826 - 836
SICI:
1617-4615(200107)265:5<826:TOMAMG>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA MISMATCH REPAIR; CROSSING-OVER; HOMEOLOGOUS RECOMBINATION; HOLLIDAY JUNCTIONS; BUDDING YEAST; CELL-CYCLE; IN-VIVO; PROTEINS; CHROMOSOME; EXPRESSION;
Keywords:
meiosis; DNA mismatch repair; MSH2; SPO21; transcriptional regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Kramer, W Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Genet & PraparatMol Biol,Grisebachstr 8, D-37077 Gottingen, Germany Univ Gottingen Grisebachstr 8 Gottingen Germany D-37077 Germany
Citazione:
C. Meyer et al., "Transcription of mutS- and mutL-homologous genes during meiosis in Saccharomyces cerevisiae and identification of a regulatory cis-element for meiotic induction of MSH2", MOL GENET G, 265(5), 2001, pp. 826-836

Abstract

We have analysed the levels of mRNA transcripts of the mutS- and mutL-homologous genes of the yeast Saccharomyces cerevisiae during the course of meiosis. by quantitative RT-PCR. We found that all mutS homologues (MSH1-6) were induced during meiosis, whereas no evidence for regulation of the mutL homologues (PMS1, MLH1-3) was obtained. Temporal expression patterns indicative of co-regulation were observed for the gene pairs MSH4/MSH5 and MSH2/SPO11. Sequence comparisons of the 5 ' flanking regions revealed similar sequence stretches in the respective gene pairs, which may constitute regulatory elements. Similar sequences were also found in the 5 ' flanking regions of the pairs MSH1/MSH3 and MSH1/MSH6. Upstream of MSH2 three closely spaced sequences similar to UAS(H) elements were found, which - surprisingly - arelocated within the coding region of SPO21. Deletion of these elements resulted in loss of meiotic induction of MSH2. Genetic analysis of homozygous deletion mutants did not reveal any differences from wild type with respect to genetic distance estimates, aberrant segregation, or suppression of homoeologus recombination in an interspecies cross with Saccharomyces paradoxus.

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Documento generato il 05/07/20 alle ore 00:29:05