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Titolo:
Identification of paralogous HERV-K LTRs on human chromosomes 3, 4, 7 and 11 in regions containing clusters of olfactory receptor genes
Autore:
Nadezhdin, EV; Lebedev, YB; Glazkova, DV; Bornholdt, D; Arman, IP; Grzeschik, KH; Hunsmann, G; Sverdlov, ED;
Indirizzi:
Russian Acad Sci, Lab Human Genes Struct & Funct, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia Russian Acad Sci Moscow Russia 117871 oorgan Chem, Moscow 117871, Russia Russian Acad Sci, Inst Mol Genet, Dept Yeast Mol Genet, Moscow 123182, Russia Russian Acad Sci Moscow Russia 123182 t Mol Genet, Moscow 123182, Russia Univ Marburg, Dept Gen Human Genet, Med Ctr Human Genet, D-35037 Marburg, Germany Univ Marburg Marburg Germany D-35037 man Genet, D-35037 Marburg, Germany German Primate Ctr, Dept Virol & Immunol, D-37077 Gottingen, Germany German Primate Ctr Gottingen Germany D-37077 D-37077 Gottingen, Germany
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 5, volume: 265, anno: 2001,
pagine: 820 - 825
SICI:
1617-4615(200107)265:5<820:IOPHLO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ENDOGENOUS RETROVIRUSES; HUMAN GENOME; HUMAN-CHROMOSOME-19; FAMILY;
Keywords:
human endogenous retroviruses; HERV-K; human genome evolution; divergence calculations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Nadezhdin, EV Russian Acad Sci, Lab Human Genes Struct & Funct, Shemyakin Ovchinnikov Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117871, Russia Russian Acad Sci Miklukho Maklaya 16-10 Moscow Russia 117871
Citazione:
E.V. Nadezhdin et al., "Identification of paralogous HERV-K LTRs on human chromosomes 3, 4, 7 and 11 in regions containing clusters of olfactory receptor genes", MOL GENET G, 265(5), 2001, pp. 820-825

Abstract

A locus harboring a human endogenous retroviral LTR (long terminal repeat)was mapped on the short arm of human chromosome 7 (7p22), and its evolutionary history was investigated. Sequences of two human genome fragments thatwere homologous to the LTR-flanking sequences were found in human genome databases: (1) an LTR-containing DNA fragment from region 3p13 of the human genome, which includes clusters of olfactory receptor genes and pseudogenes; and (2) a fragment of region 21q22.1 lacking LTR sequences. PCR analysis demonstrated that LTRs with highly homologous flanking sequences could be found in the genomes of human, chimp, gorilla, and orangutan, but were absent from the genomes of gibbon and New World monkeys. A PCR assay with a primer set corresponding to the sequence from human Chr 3 allowed us to detect LTR-containing paralogous sequences on human chromosomes 3, 4, 7, and 11. The divergence times for the LTR-flanking sequences on chromosomes 3 and 7, and the paralogous sequence on chromosome 21, were evaluated and used to reconstruct the order of duplication events and retroviral insertions. (1) Aninitial duplication event that occurred 14-17 Mya - and before LTR insertion - produced two loci, one corresponding to that located on Chr 21, while the second was the ancestor of the loci on chromosomes 3 and 7. (2) Insertion of the LTR (most probably as a provirus) into this ancestral locus took place 13 Mya. (3) Duplication of the LTR-containing ancestral locus occurred I I Mya, forming the paralogous modem loci on Chr 3 and 7.

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Documento generato il 04/07/20 alle ore 14:00:57