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Titolo:
Drosophila RNase H1 is essential for development but not for proliferation
Autore:
Filippov, V; Filippova, M; Gill, SS;
Indirizzi:
Univ Calif Riverside, Dept Cell Biol & Neurosci, Grad Program Biochem & Mol Biol, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 Biol, Riverside, CA 92521 USA Univ Calif Riverside, Dept Cell Biol & Neurosci, Grad Program Genet, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 enet, Riverside, CA 92521 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 5, volume: 265, anno: 2001,
pagine: 771 - 777
SICI:
1617-4615(200107)265:5<771:DRHIEF>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; GENETIC-ANALYSIS; MELANOGASTER; HI; IDENTIFICATION; HOMOLOG; PURIFICATION; EXPRESSION; INITIATION; CHROMOSOME;
Keywords:
Drosophila melanogaster; RNase H1; rnh1 gene; DNA replication;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Gill, SS Univ Calif Riverside, Dept Cell Biol & Neurosci, Grad Program Biochem & Mol Biol, 5419 Boyce Hall, Riverside, CA 92521 USA Univ Calif Riverside 5419 Boyce Hall Riverside CA USA 92521 1 USA
Citazione:
V. Filippov et al., "Drosophila RNase H1 is essential for development but not for proliferation", MOL GENET G, 265(5), 2001, pp. 771-777

Abstract

Ribonucleases H (RNases H) recognize and specifically degrade RNA that is bound to complementary DNA and are thought to be involved in DNA replication and transcriptional regulation. Though it was previously shown that bacterial RNases H participate in DNA synthesis, none of the known mutations in RNase H genes in either prokaryotes or lower eukaryotes is lethal. Here, wereport the characterization of the first loss-of-function mutation in an RNase Hl gene in a metazoan organism, Drosophila melanogaster. Genetic studies of this mutant showed that this gene is essential for metamorphosis in Drosophila. However, disruption of the RNase HI gene does not affect proliferation, but probably alters the regulation of gene expression. The lethal phenotype of this mutant also demonstrates that RNase HI activity in Drosophila cannot be provided by other cellular RNase H activities. Analysis of the developmental and spatial expression profiles of a reporter gene placed under the control of the RNase HI promoter revealed increased expression in several larval tissues. In salivary glands this increase was shown to be inducible by treatment with ecdysone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 15:03:16