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Titolo:
Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP
Autore:
Wu, K; Malik, K; Tian, L; Hu, M; Martin, T; Foster, E; Brown, D; Miki, B;
Indirizzi:
Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada Agr & Agri Food Canada Ottawa ON Canada K1A 0C6 ttawa, ON K1A 0C6, Canada Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, Ottawa, ON N5V 4T3, Canada Agr & Agri Food Canada Ottawa ON Canada N5V 4T3 ttawa, ON N5V 4T3, Canada
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 5, volume: 265, anno: 2001,
pagine: 763 - 770
SICI:
1617-4615(200107)265:5<763:EACPEA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; IN-VITRO; REGULATORY ELEMENTS; TRANSGENIC PLANTS; BINDING-PROTEINS; EXPRESSION; TRANSCRIPTION; ARABIDOPSIS; REQUIREMENTS; INITIATION;
Keywords:
cryptic promoter; tCUP; enhancers; core promoter; transgenic plant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Miki, B Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada Agr & Agri Food Canada Ottawa ON Canada K1A 0C6 N K1A 0C6, Canada
Citazione:
K. Wu et al., "Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP", MOL GENET G, 265(5), 2001, pp. 763-770

Abstract

Cryptic gene regulatory elements are, sequences that are inactive at theirnative locations in the genome but have the ability to become functional when positioned adjacent to genes. We have recently isolated such a cryptic sequence from tobacco, tCUP, that can act as a promoter. A 135-bp fragment spanning extending from position -197 to -62, relative to the transcriptionstart site, was found to promote GUS expression in all of the major organsof transgenic Arabidopsis plants. Furthermore, this 135-bp fragment complemented the -46 minimal promoter of CaMV 35S and conferred constitutive expression on transgenic Arabidopsis plants. An electrophoretic mobility-shift assay showed that nuclear proteins prepared from tobacco leaves interact with the 135-bp fragment. tCUP has a core promoter that lacks the TATA consensus sequence but addition of a TATA-box sequence increased the core promoter activity by three-fold. The sequence surrounding the transcription start site of tCUP has sequence similarity with the initiator element (Inr), and deletion of this sequence significantly reduced promoter activity, suggesting that an essential Inr element may exist in the tCUP core promoter. Fusion of the GCC-box enhancer element from pathogenesis-related genes to the core promoter elevated tCUP core promoter activity. Our study indicates that cryptic promoters are similar in composition and organization to promoters associated with expressed genes and that their promoter elements can be combined to create composite promoters that are fully functional. This data provides direct evidence that the expression pattern of plant genes can be influenced by cryptic gene regulatory elements when they are brought into juxtaposition with genes through DNA rearrangements.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:47:04