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Titolo:
Selective formation of Sed5p-containing SNARE complexes is mediated by combinatorial binding interactions
Autore:
Tsui, MMK; Tai, WCS; Banfield, DK;
Indirizzi:
Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol Kowloon Hong Kong Peoples R China s R China
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 3, volume: 12, anno: 2001,
pagine: 521 - 538
SICI:
1059-1524(200103)12:3<521:SFOSSC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-SNARE; MEMBRANE-FUSION; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; TRANSPORT VESICLES; GOLGI-APPARATUS; PROTEIN; YEAST; SYNTAXIN; EXOCYTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Banfield, DK Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China Hong Kong Univ Sci & Technol Kowloon Hong Kong Peoples R China
Citazione:
M.M.K. Tsui et al., "Selective formation of Sed5p-containing SNARE complexes is mediated by combinatorial binding interactions", MOL BIOL CE, 12(3), 2001, pp. 521-538

Abstract

Sed5p is the only syntaxin family member required for protein transport through the yeast Golgi and it is known to bind up to nine other soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins in vivo. We describe in vitro binding experiments in which we identify ternary and quaternary Sed5p-containing SNARE complexes. The formation of SNARE complexes among these endoplasmic reticulum- and Golgi-localized proteins requiresSed5p and is syntaxin-selective. In addition, Sed5p-containing SNARE complexes form selectively and this selectivity is mediated by Sed5p-containing intermediates that discriminate among subsequent binding partners. Althoughmany of these SNAREs have overlapping distributions in vivo, the SNAREs that form complexes with Sed5p in vitro reflect their functionally distinct locales. Although SNARE-SNARE interactions are promiscuous and a single SNARE protein is often found in more than one complex, both the biochemical as well as genetic analyses reported here suggest that this is not a result ofnonselective direct substitution of one SNARE for another. Rather our dataare consistent with the existence of multiple (perhaps parallel) trafficking pathways where Sed5p-containing SNARE complexes play overlapping and/or distinct functional roles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:47:39