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Titolo:
Nonsense-mediated decay of mRNA for the selenoprotein phospholipid hydroperoxide glutathione peroxidase is detectable in cultured cells but masked orinhibited in rat tissues
Autore:
Sun, XL; Li, XJ; Moriarty, PM; Henics, T; LaDuca, JP; Maquat, LE;
Indirizzi:
Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA Roswell Pk Canc Inst Buffalo NY USA 14263 nc Genet, Buffalo, NY 14263 USA Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 & Biophys, Rochester, NY 14642 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 4, volume: 12, anno: 2001,
pagine: 1009 - 1017
SICI:
1059-1524(200104)12:4<1009:NDOMFT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA STABILITY; SELENIUM DEFICIENCY; GENE-EXPRESSION; TRANSLATION TERMINATION; 3'-UNTRANSLATED REGION; MAMMALIAN-CELLS; SURVEILLANCE; INTRON; CODON; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Maquat, LE Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA Roswell Pk Canc Inst Buffalo NY USA 14263 uffalo, NY 14263 USA
Citazione:
X.L. Sun et al., "Nonsense-mediated decay of mRNA for the selenoprotein phospholipid hydroperoxide glutathione peroxidase is detectable in cultured cells but masked orinhibited in rat tissues", MOL BIOL CE, 12(4), 2001, pp. 1009-1017

Abstract

Previous studies of mRNA for classical glutathione peroxidase I (GPx1) demonstrated that hepatocytes of rats fed a selenium-deficient diet have less cytoplasmic GPx1 mRNA than hepatocytes of rats fed a selenium-adequate diet. This is because GPx1 mRNA is degraded by the surveillance pathway called nonsense-mediated mRNA decay (NMD) when the selenocysteine codon is recognized as nonsense. Here, we examine the mechanism by which the abundance of phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA, another selenocysteine-encoding mRNA, fails to decrease in the hepatocytes and testicular cells of rats fed a selenium-deficient diet. We demonstrate with cultured NIH3T3 fibroblasts or H35 hepatocytes transiently transfected with PHGPx gene variants under selenium-supplemented or selenium-deficient conditions that PHGPx mRNA is, in fact, a substrate for NMD when the selenocysteine codon is recognized as nonsense. We also demonstrate that the endogenous PHGPx mRNA of untransfected H35 cells is subject to NMD. The failure of previous reports to detect the NMD of PHGPx mRNA in cultured cells is likely attributable to the expression of PHGPx cDNA rather than the PHGPx gene. We conclude that 1) the sequence of the PHGPx gene is adequate to support the NMD of product mRNA, and 2) there is a mechanism in liver and testis but not cultured fibroblasts and hepatocytes that precludes or masks the NMD of PHGPxmRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:57:29