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Titolo:
Dynamics of immature secretory granules: Role of cytoskeletal elements during transport, cortical restriction, and F-actin-dependent tethering
Autore:
Rudolf, R; Salm, T; Rustom, A; Gerdes, HH;
Indirizzi:
Univ Heidelberg, Dept Neurobiol, Interdisciplinary Ctr Neurosci, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 5, volume: 12, anno: 2001,
pagine: 1353 - 1365
SICI:
1059-1524(200105)12:5<1353:DOISGR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; AP-1 ADAPTER COMPLEX; CELL-FREE SYSTEM; REAL-TIME; CA2+-ACTIVATED EXOCYTOSIS; GOLGI NETWORK; PC12 CELLS; IN-VIVO; VESICLES; VISUALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Gerdes, HH Univ Heidelberg, Dept Neurobiol, Interdisciplinary Ctr Neurosci, Neuenheimer Feld 364, D-69120 Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 364 Heidelberg Germany D-69120
Citazione:
R. Rudolf et al., "Dynamics of immature secretory granules: Role of cytoskeletal elements during transport, cortical restriction, and F-actin-dependent tethering", MOL BIOL CE, 12(5), 2001, pp. 1353-1365

Abstract

Secretory granules store neuropeptides and hormones and exhibit regulated exocytosis upon appropriate cellular stimulation. They axe generated in thetrans-Golgi network as immature secretory granules, short-lived vesicular intermediates, which undergo a complex and poorly understood maturation process. Due to their short half-life and low abundance, real-time studies of immature secretory granules have not been previously possible. We describe here a pulse/chase-like system based on the expression of a human chromogranin B-GFP fusion protein in neuroendocrine PC12 cells, which permits directvisualization of the budding of immature secretory granules and their dynamics during maturation. Live cell imaging revealed that newly formed immature secretory granules are transported in a direct and microtubule-dependentmanner within a few seconds to the cell periphery. Our data suggest that the cooperative action of microtubules and actin filaments restricts immature secretory granules to the F-actin-rich cell cortex, where they move randomly and mature completely within a few hours. During this maturation period, secretory granules segregate into pools of different motility. In a late phase of maturation, 60% of secretory granules were found to be immobile and about half of these underwent F-actin-dependent tethering.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 13:09:44