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Titolo:
Regulation of initiation of S phase, replication checkpoint signaling, andmaintenance of mitotic chromosome structures during S phase by Hsk1 kinasein the fission yeast
Autore:
Takeda, T; Ogino, K; Tatebayashi, K; Ikeda, H; Arai, K; Masai, H;
Indirizzi:
Univ Tokyo, Inst Med Sci, Dept Mol & Dev biol, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Dept Mol & Dev biol, Tokyo 1088639, Japan Univ Tokyo, Inst Med Sci, Dept Biol Mol, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Sci, Dept Biol Mol, Tokyo 1088639, Japan Kitasato Inst, Tokyo 1080072, Japan Kitasato Inst Tokyo Japan 1080072Kitasato Inst, Tokyo 1080072, Japan Japan Sci & Technol Corp, Core Res Engn Sci & Technol, Tokyo 1088639, Japan Japan Sci & Technol Corp Tokyo Japan 1088639 chnol, Tokyo 1088639, Japan Tokyo Metropolitan Inst Med Sci, Dept Cell Biol, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613, Japan
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 5, volume: 12, anno: 2001,
pagine: 1257 - 1274
SICI:
1059-1524(200105)12:5<1257:ROIOSP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDC7 PROTEIN-KINASE; SISTER-CHROMATID COHESION; SACCHAROMYCES-CEREVISIAE CDC7; HUMAN CDC7-RELATED KINASE; IN-VITRO PHOSPHORYLATION; CELL-CYCLE REGULATION; DNA-REPLICATION; SCHIZOSACCHAROMYCES-POMBE; BUDDING YEAST; G(1)/S TRANSITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Takeda, T Univ Tokyo, Inst Med Sci, Dept Mol & Dev biol, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Dev biol, Tokyo 1088639, Japan
Citazione:
T. Takeda et al., "Regulation of initiation of S phase, replication checkpoint signaling, andmaintenance of mitotic chromosome structures during S phase by Hsk1 kinasein the fission yeast", MOL BIOL CE, 12(5), 2001, pp. 1257-1274

Abstract

Hsk1, Saccharomyces cerevisiae Cdc7-related kinase in Shizosaccharomyces pombe, is required for G1/S transition and its kinase activity is controlledby the regulatory subunit Dfp1/Him1. Analyses of a newly isolated temperature-sensitive mutant, hsk1-89, reveal that Hsk1 plays crucial roles in DNA replication checkpoint signaling and maintenance of proper chromatin structures during mitotic S phase through regulating the functions of Rad3 (ATM)-Cds1 and Rad21 (cohesin), respectively, in addition to expected essential roles for initiation of mitotic DNA replication through phosphorylating Cdc19 (Mcm2). Checkpoint defect in hsk1-89 is indicated by accumulation of cut cells at 30 degreesC. hsk1-89 displays synthetic lethality in combination with rad3 deletion, indicating that survival of hsk1-89 depends on Rad3-dependent checkpoint pathway. Cds1 kinase activation, which normally occurs in response to early S phase arrest by nucleotide deprivation, is largely impaired in hsk1-89. Furthermore, Cds1-dependent hyperphosphorylation of Dfp1 in response to hydroxyurea arrest is eliminated in hsk1-89, suggesting that sufficient activation of Hsk1-Dfp1 kinase is required for S phase entry andreplication checkpoint signaling. hsk1-89 displays apparent defect in mitosis at 37 degreesC leading to accumulation of cells with near 2C DNA content and with aberrant nuclear structures. These phenotypes are similar to those of rad21-K1 and are significantly enhanced in a hsk1-89 rad21-K1 double mutant. Consistent with essential roles of Rad21 as a component for the cohesin complex, sister chromatid cohesion is partially impaired in hsk1-89, suggesting a possibility that infrequent origin firing of the mutant may affect the cohesin functions during S phase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 08:40:37