Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Yeast Rab GTPase-activating protein Gyp1p localizes to the Golgi apparatusand is a negative regulator of Ypt1p
Autore:
Du, LL; Novick, P;
Indirizzi:
Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 ophys & Biochem, New Haven, CT 06520 USA Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA Yale Univ NewHaven CT USA 06520 Dept Cell Biol, New Haven, CT 06520 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 5, volume: 12, anno: 2001,
pagine: 1215 - 1226
SICI:
1059-1524(200105)12:5<1215:YRGPGL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; VESICULAR TRANSPORT; NUCLEOTIDE EXCHANGE; ACTIN CYTOSKELETON; VESICLE DOCKING; CIS-GOLGI; GAP; DOMAIN; IDENTIFICATION; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Novick, P Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 ochem, New Haven, CT 06520 USA
Citazione:
L.L. Du e P. Novick, "Yeast Rab GTPase-activating protein Gyp1p localizes to the Golgi apparatusand is a negative regulator of Ypt1p", MOL BIOL CE, 12(5), 2001, pp. 1215-1226

Abstract

A family of related proteins in yeast Saccharomyces cerevisiae is known tohave in vitro GTPase-activating protein activity on the Rab GTPases. However, their in vivo function remains obscure. One of them, Gyp1p, acts on Sec4p, Ypt1p, Ypt7p, and Ypt51p in vitro. Here, we present data to reveal its in vivo substrate and the role that it plays in the function of the Rab GTPase. Red fluorescent protein-tagged Gyp1p is concentrated on cytoplasmic punctate structures that largely colocalize with a cis-Golgi marker. Subcellular fractionation of a yeast lysate confirmed that Gyp1p is peripherally associated with membranes and that it cofractionates with Golgi markers. Thislocalization suggests that Gyp1p may only act on Rab GTPases on the Golgi. A gyp1 Delta strain displays a growth defect on synthetic medium at 37 degreesC. Overexpression of Ypt1p, but not other Rab GTPases, strongly inhibits the growth of gyp1 Delta cells. Conversely, a partial loss-of-function allele of YPT1, ypt1-2, can suppress the growth defect of gyp1 Delta cells. Furthermore, deletion of GYP1 can partially suppress growth defects associated with mutants in subunits of transport protein particle complex, a complex that catalyzes nucleotide exchange on Ypt1p. These results establish thatGyp1p functions on the Golgi as a negative regulator of Ypt1p.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:50:53