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Titolo:
Homotypic fusion of immature secretory granules during maturation requiressyntaxin 6
Autore:
Wendler, F; Page, L; Urbe, S; Tooze, SA;
Indirizzi:
Imperial Canc Res Fund, Secretory Pathway Lab, London WC2A 3PX, England Imperial Canc Res Fund London England WC2A 3PX London WC2A 3PX, England
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 6, volume: 12, anno: 2001,
pagine: 1699 - 1709
SICI:
1059-1524(200106)12:6<1699:HFOISG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; ADRENAL CHROMAFFIN CELLS; ADAPTER PROTEIN AP-1; MEMBRANE-FUSION; T-SNARE; SECRETOGRANIN-II; NEUROENDOCRINE CELLS; VESICLE TRAFFICKING; ENDOPEPTIDASE PC2; RAB5 EFFECTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Tooze, SA Imperial Canc Res Fund, Secretory Pathway Lab, 44 Lincolns Inn Fields, London WC2A 3PX, England Imperial Canc Res Fund 44 Lincolns Inn Fields London England WC2A 3PX
Citazione:
F. Wendler et al., "Homotypic fusion of immature secretory granules during maturation requiressyntaxin 6", MOL BIOL CE, 12(6), 2001, pp. 1699-1709

Abstract

Homotypic fusion of immature secretory granules (ISGs) gives rise to mature secretory granules (MSGs), the storage compartment in endocrine and neuroendocrine cells for hormones and neuropeptides. With the use of a cell-freefusion assay, we investigated which soluble N-ethylmaleimide-sensitive fusion protein attachment receptor (SNARE) molecules are involved in the homotypic fusion of ISGs. Interestingly, the SNARE molecules mediating the exocytosis of MSGs in neuroendocrine cells, syntaxin 1, SNAP-25, and VAMP2, werenot involved in homotypic ISG fusion. Instead, we have identified syntaxin6 as a component of the core machinery responsible for homotypic ISG fusion. Subcellular fractionation studies and indirect immunofluorescence microscopy show that syntaxin 6 is sorted away during the maturation of ISGs to MSGs. Although, syntaxin 6 on ISG membranes is associated with SNAP-25 and SNAP-29/GS32, we could not find evidence that these target (t)-SNARE molecules are involved in homotypic ISG fusion. Nor could we find any involvement for the vesicle (v)-SNARE VAMP4, which is known to be associated with syntaxin 6. Importantly, we have shown that homotypic fusion requires the function of syntaxin 6 on both donor as well as acceptor membranes, which suggests that t-t-SNARE interactions, either direct or indirect, may be required during fusion of ISG membranes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:01:19