Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of a retrotransposon-like element from Entamoeba histolytica
Autore:
Sharma, R; Bagchi, A; Bhattacharya, A; Bhattacharya, S;
Indirizzi:
Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India Jawaharlal Nehru Univ New Delhi India 110067 ci, New Delhi 110067, India Jawaharlal Nehru Univ, Sch Environm Sci, New Delhi 110067, India Jawaharlal Nehru Univ New Delhi India 110067 ci, New Delhi 110067, India
Titolo Testata:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
fascicolo: 1, volume: 116, anno: 2001,
pagine: 45 - 53
SICI:
0166-6851(200108)116:1<45:COAREF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENES; DNA; SEQUENCES; PROTEIN; REPEATS; IDENTIFICATION; TRANSPOSITION; DROSOPHILA; COPY;
Keywords:
Entamoeba histolytica; non-long terminal repeat; retrotransposons; reverse transcriptase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Bhattacharya, A Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India Jawaharlal Nehru Univ New Delhi India 110067 0067, India
Citazione:
R. Sharma et al., "Characterization of a retrotransposon-like element from Entamoeba histolytica", MOL BIOCH P, 116(1), 2001, pp. 45-53

Abstract

The protozoan parasite Entamoeba histolytica is the causative agent of amoebiasis. The genome organization of this organism is not well understood. We had earlier reported the presence of a multicopy sequence, HMc, in E. histolytica. Subsequent analysis showed that HMc is a member of a retrotransposon family that we have named the E. histolytica retrotransposon-like element (EhRLE). Four other members of this family have been characterized. The EhRLE family is distributed across all chromosomes of the parasite. There are 140 copies, which show minor sequence variation with respect to one another (2-4% from the consensus sequence). From a sequence analysis of five members of the EhRLE family, the complete EhRLE unit is estimated to be 4086 bp in length. It has a 27-mer inverted repeat at its ends. A pairwise comparison with sequences in the database showed a highly significant match of apart of EhRLE with reverse transcriptases (RT), especially those encoded by non-long terminal repeat retrotransposons. There are stop codons in all the five EhRLEs, but a continuous open reading frame of 464 amino acids could be reconstructed by comparing the sequences of several EhRLEs. The reconstructed sequence showed a much better identity with RT as compared with anyof the original EhRLE sequences. The non-pathogenic species, Entamoeba dispar, also contains this element, with 85% sequence identity with EhRLE. Thedata suggest that EhRLE may be a retrotransposon, but many of its members are probably nonfunctional due to the accumulation of mutations. (C) 2001 Elsevier Science BY. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:46:27