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Titolo:
A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses
Autore:
Bello, FJ; Rodriguez, JA; Escovar, J; Olano, VA; Morales, A; Gonzalez, M; Rey, G;
Indirizzi:
Univ De La Salle, Dept Biol & Quim, Bogota, DC, Colombia Univ De La SalleBogota DC Colombia pt Biol & Quim, Bogota, DC, Colombia Inst Nacl Salud, Virol Lab, Bogota, DC, Colombia Inst Nacl Salud Bogota DC Colombia alud, Virol Lab, Bogota, DC, Colombia Inst Nacl Salud, Entomol Lab, Bogota, DC, Colombia Inst Nacl Salud BogotaDC Colombia ud, Entomol Lab, Bogota, DC, Colombia
Titolo Testata:
MEMORIAS DO INSTITUTO OSWALDO CRUZ
fascicolo: 6, volume: 96, anno: 2001,
pagine: 865 - 873
SICI:
0074-0276(200108)96:6<865:ANCCLF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENEZUELAN EQUINE ENCEPHALITIS; LUTZOMYIA-LONGIPALPIS DIPTERA; ESTABLISHMENT; PSYCHODIDAE; COLOMBIA; VIRUSES;
Keywords:
Psorophora confinnis; cell line; chromosomes; isozymes; arbovirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Bello, FJ Univ De La Salle, Dept Biol & Quim, Carrera 2A,10-70, Bogota, DC, Colombia Univ De La Salle Carrera 2A,10-70 Bogota DC Colombia , Colombia
Citazione:
F.J. Bello et al., "A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses", MEM I OSW C, 96(6), 2001, pp. 865-873

Abstract

A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora. confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:44:24