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Titolo:
A simple modification of the Baermann method for diagnosis of Strongyloidiasis
Autore:
Hernandez-Chavarria, F; Avendano, L;
Indirizzi:
Univ Costa Rica, Unidad Microscopia Elect, San Jose, Costa Rica Univ CostaRica San Jose Costa Rica oscopia Elect, San Jose, Costa Rica Univ Costa Rica, Fac Microbiol, San Jose, Costa Rica Univ Costa Rica San Jose Costa Rica Fac Microbiol, San Jose, Costa Rica
Titolo Testata:
MEMORIAS DO INSTITUTO OSWALDO CRUZ
fascicolo: 6, volume: 96, anno: 2001,
pagine: 805 - 807
SICI:
0074-0276(200108)96:6<805:ASMOTB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGAR PLATE METHOD; STERCORALIS; INFECTION;
Keywords:
Strongyloides stercoralis; Baermann method; agar culture plate method; direct smear; intestinal parasites;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Hernandez-Chavarria, F Univ Costa Rica, Unidad Microscopia Elect, San Jose, Costa Rica Univ Costa Rica San Jose Costa Rica , Costa Rica
Citazione:
F. Hernandez-Chavarria e L. Avendano, "A simple modification of the Baermann method for diagnosis of Strongyloidiasis", MEM I OSW C, 96(6), 2001, pp. 805-807

Abstract

The diagnosis of Strongyloides stercoralis infections is routinely made bymicroscopic observation of larvae in stool samples, a low sensitivity method, or by other, most effective methods, such as the Baermann or agar culture plate methods. We propose in this paper a practical modification of Baermann method. One hundred and six stool samples from alcoholic patients were analyzed using the direct smear test, agar culture plate method, the standard Baermannmethod, and its proposed modification. For this modification the funnel used in the original version of the method is substituted by a test tube witha rubber stopper, perforated to allow insertion of a pipette tip. The tubewith a fecal suspension is inverted over another tube containing 6 ml of saline solution and incubated at 37 degreesC for at least 2 h. The saline solution from the second tube is centrifuged and the pellet is observed microscopically. Larva of S. stercoralis were detected in six samples (5.7%) by the two versions of the Baermann method. Five samples were positive using the agar culture plate method, and only in two samples the larva were observed using direct microscopic observation of fecal smears. Cysts of Endolimax nana and Entamoeba histolytica/dyspar were also detected in the modification of Baermann method. Data obtained by the modified Baermann method suggest that this methodology may helps concentrate larvae of S. stercoralis as efficiently as the original method.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:21:25