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Titolo:
Leishmania (Leishmania) major-infected rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
Autore:
Amaral, VF; Teva, A; Porrozzi, R; Silva, AJ; Pereira, MS; Oliveira-Neto, MP; Grimaldi, G;
Indirizzi:
FIOCRUZ, Inst Oswaldo Cruz, Dept Immunol, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil FIOCRUZ, Inst Oswaldo Cruz, Dept Ultraestrut & Biol Celular, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil FIOCRUZ, Hosp Evandro Chagas, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil
Titolo Testata:
MEMORIAS DO INSTITUTO OSWALDO CRUZ
fascicolo: 6, volume: 96, anno: 2001,
pagine: 795 - 804
SICI:
0074-0276(200108)96:6<795:L(MRM(>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMERICAN CUTANEOUS LEISHMANIASIS; IMMUNOGLOBULIN-G SUBCLASSES; HUMORAL IMMUNE-RESPONSES; INDIAN KALA-AZAR; VISCERAL LEISHMANIASIS; T-CELL; SAIMIRI-SCIUREUS; LESIONS; MONKEYS; BRAZILIENSIS;
Keywords:
rhesus macaques; Macaca mulatta; nonhuman primates; experimental leishmaniasis; Leishmania (L.) major; immune responses; histopathology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Grimaldi, G FIOCRUZ, Inst Oswaldo Cruz, Dept Immunol, Av Brasil 4365, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Av Brasil 4365 Rio De Janeiro Brazil BR-21045900 BCil
Citazione:
V.F. Amaral et al., "Leishmania (Leishmania) major-infected rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections", MEM I OSW C, 96(6), 2001, pp. 795-804

Abstract

Seven rhesus macaques were infected intradermally with 10(7) promastigotesof Leishmania (Leishmania) major. All monkeys developed a localized, ulcerative, self-healing nodular skin lesion at the site of inoculation of the parasite. Non-specific chronic inflammation and/or tuberculoid-type granulomatous reaction were the main histopathological manifestations of the disease. Serum Leishmania-specific antibodies (IgG and IgGl) were detected by ELISA in all infected animals; immunoblot analyses indicated that numerous antigens were recognized. A very high degree of variability was observed in the parasite-specific cell-mediated immune responses [as detected by measuring delated-type hypersensitivity (DTH) reaction, in vitro lymphocyte proliferation, and gamma interferon (IFN-gamma) production] for individuals over time post challenge. From all the recovered monkeys (which showed resolutionof the lesions after 11 weeks of infection), 57.2% (4/7) and 28.6% (2/7) animals remained susceptible to secondary and tertiary infections, respectively, but the disease severity was altered (i.e. lesion size was smaller andhealed faster than in the primary infection). The remaining monkeys exhibited complete resistance (i.e. no lesion) to each rechallenge. Despite the inability to consistently detect correlates of cell-Mediated immunity to Leishmania or correlation between resistance to challenge and DTH, lymphocyte transformation or IFN-gamma production, partial or complete acquired resistance was conferred by experimental infection. This primate model should be useful for measuring vaccine effectiveness against the human disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:22:08