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Titolo:
T-cell clones derived by CD3 stimulation from hepatitis C virus explanted liver tissue are not representative of dominant clones present in vivo
Autore:
Davey, MP; Rosen, HR; Woody, CN; Haley, DP; Kurkinen, J; Lewinsohn, DM;
Indirizzi:
Dept Vet Affairs Med Ctr, Portland, OR 97201 USA Dept Vet Affairs Med CtrPortland OR USA 97201 tr, Portland, OR 97201 USA Oregon Hlth Sci Univ, Dept Med, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 pt Med, Portland, OR 97201 USA Oregon Hlth Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97201 USAOregon Hlth Sci Univ Portland OR USA 97201 mmunol, Portland, OR 97201 USA
Titolo Testata:
LIVER TRANSPLANTATION
fascicolo: 8, volume: 7, anno: 2001,
pagine: 716 - 723
SICI:
1527-6465(200108)7:8<716:TCDBCS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; RHEUMATOID-ARTHRITIS; NORMAL INDIVIDUALS; IMMUNE-SYSTEM; LYMPHOCYTES; POPULATIONS; REPERTOIRE; GAMMA; EXPRESSION; CLONOTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Davey, MP Dept Vet Affairs Med Ctr, 3710 SW US Vet Hosp Rd,Bldg 101,Rm 502,Mail Stop, Portland, OR 97201 USA Dept Vet Affairs Med Ctr 3710 SW US Vet Hosp Rd,Bldg 101,Rm 502,Mail Stop Portland OR USA 97201
Citazione:
M.P. Davey et al., "T-cell clones derived by CD3 stimulation from hepatitis C virus explanted liver tissue are not representative of dominant clones present in vivo", LIVER TRANS, 7(8), 2001, pp. 716-723

Abstract

Liver tissue from hepatitis C virus (HCV)-related endstage disease contains T-cell infiltrates. The goal of this study is to determine whether CD4 T-cell clones established in vitro using an antigen-independent technique from explanted liver tissue (n=3) are representative of dominant clones present in vivo. T-cell receptor (TCR) use by intrahepatic CD4 T cells was assessed by spectra-type analysis. Clones were established from single CD4 T cells by culturing in vitro with anti-CD3 and interleukin-2 (n>25 per patient). TCR genes expressed by each done were identified by sequencing. When identical clones were isolated, the original spectra-type was analyzed further to determine whether the clone was a dominant T-cell expansion in vivo. Evidence for clonal expansions was found in all patients by spectra-typing. T cells expressing the same TCRBV genes used for spectratyping were cloned in vitro. Duplicate clones expressing the same TCR genes were observed in 2 patients. Confirmation that clones established in vitro matched those presentin vivo was obtained for 2 clones. Many dominant clones identified by spectratyping did not proliferate in vitro. Although spectratyping suggested the widespread accumulation of clonal expansions in HCV-related end-stage liver disease, clones established in vitro using anti-CD3 were poorly representative of dominant clones present in vivo. Although cloning with anti-CD3 has the advantage of generating T-cell clones not biased a priori toward a specific antigen, modified cloning strategies will need to be developed to expand those clones that appear dominant in end-stage organs.

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Documento generato il 01/06/20 alle ore 00:43:09