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Titolo:
Prognostic significance of free gastrointestinal tumor cells in peritoneallavage detected by immunocytochemistry and polymerase chain reaction
Autore:
Broll, R; Weschta, M; Windhoevel, U; Berndt, S; Schwandner, O; Roblick, U; Schiedeck, THK; Schimmelpenning, H; Bruch, HP; Duchrow, M;
Indirizzi:
Univ Clin Lubeck, Surg Res Lab, D-23538 Lubeck, Germany Univ Clin Lubeck Lubeck Germany D-23538 Res Lab, D-23538 Lubeck, Germany Univ Clin Lubeck, Dept Surg, D-23538 Lubeck, Germany Univ Clin Lubeck Lubeck Germany D-23538 pt Surg, D-23538 Lubeck, Germany
Titolo Testata:
LANGENBECKS ARCHIVES OF SURGERY
fascicolo: 4, volume: 386, anno: 2001,
pagine: 285 - 292
SICI:
1435-2443(200107)386:4<285:PSOFGT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC-CANCER; CARCINOEMBRYONIC ANTIGEN; GASTRIC-CARCINOMA; MONOCLONAL-ANTIBODIES; COLORECTAL-CANCER; SEROUS EFFUSIONS; BONE-MARROW; CYTOLOGY; EXPRESSION; CEA;
Keywords:
immunocytochemistry; RT PCR; carcinoembryonic antigen; peritoneal lavage; gastrointestinal tumors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Broll, R Univ Clin Lubeck, Surg Res Lab, Ratzeburger Allee 160, D-23538 Lubeck, Germany Univ Clin Lubeck Ratzeburger Allee 160 Lubeck Germany D-23538 ny
Citazione:
R. Broll et al., "Prognostic significance of free gastrointestinal tumor cells in peritoneallavage detected by immunocytochemistry and polymerase chain reaction", LANG ARCH S, 386(4), 2001, pp. 285-292

Abstract

Aims: The aim of our study was to identify tumor cells in peritoneal lavage comparatively with immunocytochemistry (ICC) and half-nested reverse transcriptase-polymerase chain reaction (RT PCR) using carcinoembryonic antigen(CEA) as marker and to evaluate their prognostic significance. Patients and methods: In 75 patients who underwent surgery for a carcinoma of the colorectum (n=49), stomach (n=17) or pancreas (n=9) and 13 patients with an abdominal aortic aneurysm (control group) the abdomen was irrigated with saline solution immediately after laparotomy. Cells were separated by Ficoll-density centrifugation and divided into 2 equal volumes for ICC and RT PCR. For ICC cells were spun onto slides by cytospin centrifugation and stained with a monoclonal antibody (mab) against CEA using the APAAP method. For RT PCR total RNA was extracted from the cells, transcribed into cDNA and amplified with CEA-specific primers. Lavages of 13 patients with an abdominal aortic aneurysm and blood samples of 6 healthy donors served as controls. Results: Immunostained tumor cells were found in peritoneal lavage in 23% (17/75) of all patients, whereas 63% (47/75) of patients gave a positive result by RT PCR analysis. In the control group (n=13) no patient presented with tumor cells in ICC, however 5 of 13 (38%) showed amplified CEA-mRNA by RT-PCR, and so did one of six blood samples. Using ICC technique, we found significant correlations between detection rates and pT, pN-, pM-categories as well as tumor stage. On the contrary, by RT PCR significant correlations were observed only between pT- and pM-categories and detection rates. Detection of tumor cells in peritoneal lavage with both techniques was associated with poor prognosis. Moreover, these tumor cells are an independent prognostic factor and may have an influence on the development of peritoneal carcinomatosis. Conclusion: ICC is a useful method for detection of tumor cells in peritoneal lavage. In contrast, half-nested RT-PCR cannot be recommended,as the detection rates are unproportionally high, obviously as a result ofCEA-mRNA expression in nontumor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:59:25