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Titolo:
Genotypes of pestivirus RNA detected in live virus vaccines for human use
Autore:
Giangaspero, M; Vacirca, G; Harasawa, R; Buttner, M; Panuccio, A; Morghen, CD; Zanetti, A; Belloli, A; Verhulst, A;
Indirizzi:
Univ Tokyo, Grad Sch Med, Anim Ctr Biomed Res, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 omed Res, Bunkyo Ku, Tokyo 1130033, Japan Univ Milan, Fac Vet Med, Inst Special Pathol, Milan, Italy Univ Milan Milan Italy , Fac Vet Med, Inst Special Pathol, Milan, Italy Univ Milan, Fac Vet Med, Vet Med Clin, Milan, Italy Univ Milan Milan Italy v Milan, Fac Vet Med, Vet Med Clin, Milan, Italy Fed Res Ctr Virus Dis Anim, Inst Immunol, D-7400 Tubingen, Germany Fed ResCtr Virus Dis Anim Tubingen Germany D-7400 400 Tubingen, Germany Multizonal Ctr Hyg & Prevent, Milan, Italy Multizonal Ctr Hyg & Prevent Milan Italy tr Hyg & Prevent, Milan, Italy Univ Milan, Fac Med, Inst Pharmacol, Milan, Italy Univ Milan Milan Italy niv Milan, Fac Med, Inst Pharmacol, Milan, Italy Univ Milan, Fac Med, Inst Virol, Milan, Italy Univ Milan Milan ItalyUniv Milan, Fac Med, Inst Virol, Milan, Italy Inst Trop Med Prince Leopold, B-2000 Antwerp, Belgium Inst Trop Med PrinceLeopold Antwerp Belgium B-2000 000 Antwerp, Belgium
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 7, volume: 63, anno: 2001,
pagine: 723 - 733
SICI:
0916-7250(200107)63:7<723:GOPRDI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE-VIRAL-DIARRHEA; HOG-CHOLERA VIRUS; NUCLEOTIDE-SEQUENCE; MOLECULAR-CLONING; 5'-UNTRANSLATED REGION; BORDER DISEASE; CONTAMINATED VACCINE; PHYLOGENETIC ANALYSIS; CELL-CULTURES; STRAIN;
Keywords:
contamination; genotype; pestivirus; vaccine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Harasawa, R Univ Tokyo, Grad Sch Med, Anim Ctr Biomed Res, Bunkyo Ku, Hongo 7-3-1, Tokyo 1130033, Japan Univ Tokyo Hongo 7-3-1 Tokyo Japan 1130033 kyo 1130033, Japan
Citazione:
M. Giangaspero et al., "Genotypes of pestivirus RNA detected in live virus vaccines for human use", J VET MED S, 63(7), 2001, pp. 723-733

Abstract

Live virus vaccines for human use, 29 monovalent vaccines against measles,mumps, rubella or polio, eight polyvalent vaccines against measles-mumps-rubella and one bacterial polyvalent vaccine against Streptococcus pneumoniae, were tested by reverse transcriptase-nested PCR for the presence of petivirus or pestivirus RNA. Twenty-four samples were selected from European manufacturers, ten were from U.S.A. and four from Japan. Five (13.1%) out of 38 tested samples were positive for pestivirus RNA. Three vaccines (rubellaand two measles) were from Europe and two (mumps and rubella) from Japan. The 5 ' -untranslated genomic region of the contaminant pestivirus RNA wereamplified by reverse transcription-PCR and sequenced. Analyses based on primary nucleotide sequence homology and on secondary structures, characteristic to genotypes, revealed that the cDNA sequences belonged to bovine viraldiarrhea virus (BVDV). A cDNA sequence, detected from one measles sample, belonged to BVDV-1b genotype. Pestiviral cDNA detected from the Japanese mumps and rubella vaccine samples, belonged to the BVDV genotypes 1a and 1c, respectively. Analysis on two cDNA sequences detected from measles and rubella vaccine samples from Europe showed their appurtenance to a new genotype, BVDV-1d. These findings indicate that contamination by animal pestivirus may occur in biological products for human use.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 14:14:54