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Titolo:
Expression of peptide transporter following intestinal transplantation in the rat
Autore:
Motohashi, H; Masuda, S; Katsura, T; Saito, H; Sakamoto, S; Uemoto, S; Tanaka, K; Inui, K;
Indirizzi:
Kyoto Univ Hosp, Fac Med, Dept Pharm, Sakyo Ku, Kyoto 6068507, Japan KyotoUniv Hosp Kyoto Japan 6068507 harm, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ, Grad Sch Med, Dept Transplantat & Immunol, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 & Immunol, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
JOURNAL OF SURGICAL RESEARCH
fascicolo: 2, volume: 99, anno: 2001,
pagine: 294 - 300
SICI:
0022-4804(200108)99:2<294:EOPTFI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
H+/PEPTIDE COTRANSPORTER; GLUCOSE; KIDNEY; LOCALIZATION; ABSORPTION;
Keywords:
intestinal transplantation; tacrolimus; peptide transporter; rejection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Inui, K Kyoto Univ Hosp, Fac Med, Dept Pharm, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Hosp Kyoto Japan 6068507 kyo Ku, Kyoto 6068507, Japan
Citazione:
H. Motohashi et al., "Expression of peptide transporter following intestinal transplantation in the rat", J SURG RES, 99(2), 2001, pp. 294-300

Abstract

Background. The absorptive function of the intestinal graft is one of the most important factors for successful intestinal transplantation. To clarify whether the intestinal H+/peptide cotransporter (PEPT1) was expressed in the transplanted intestine, we examined the expression of PEPT1 in an experimental model of rat small intestinal transplantation in comparison with expression of Na+/glucose cotransporter (SGLT1). Materials and methods. Heterotopic intestinal transplantation was performed in allogeneic and syngeneic rat strain combinations. An additional group of allogeneic recipients was treated with tacrolimus (1 mg/kg) prior to transplantation, then daily for 7 days. Intestinal grafts were examined for histopathology and PEPT1 and SGLT1 expression. Results. In the isografts, the levels of messenger RNA (mRNA) encoding both transporters were not changed, while the amount of SGLT1 protein was decreased and that of PEPT1 protein was increased. In the allografts, mRNA level and protein amount of both transporters and the amount of villin protein were decreased, and microscopic examination revealed histopathological features of rejection on day 7. Tacrolimus treatment ameliorated the histopathological features and prevented the decrease in villin protein expression. However, the decreases in PEPT1 and SGLT1 expression (both mRNA and protein)were partially prevented by tacrolimus treatment. Conclusions. This study indicated that the expression of transporters should be determined to evaluate intestinal graft function in addition to histopathological examination of the mucosa and that the levels of mRNA encodingintestinal nutrient transporters in biopsy specimens may be useful for evaluating the intestinal graft function for intestinal transplant patients. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:21:45