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Titolo:
Crystallization and preliminary X-ray studies of V-1-ATPase of Thermus thermophilus HB8 complexed with Mg-ADP
Autore:
Ishii, N; Saijo, S; Sato, T; Tanaka, N; Harata, K;
Indirizzi:
Natl Inst Biosci & Human Technol, Biophys Chem Lab, Tsukuba, Ibaraki 3058566, Japan Natl Inst Biosci & Human Technol Tsukuba Ibaraki Japan 3058566 566, Japan Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, Kanagawa 2268501, Japan Tokyo Inst Technol Kanagawa Japan 2268501 ri Ku, Kanagawa 2268501, Japan
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 1, volume: 134, anno: 2001,
pagine: 88 - 92
SICI:
1047-8477(200104)134:1<88:CAPXSO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE HEART-MITOCHONDRIA; MEMBRANE-BOUND ATPASE; PURIFICATION; F1-ATPASE; ARCHAEBACTERIUM; EUBACTERIUM; F-1-ATPASE; SYNTHASE; SUBUNIT; PUMPS;
Keywords:
V-1-ATPase; Thermus thermophilus; X-ray crystallography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ishii, N Natl Inst Adv Ind Sci & Technol, Biol Informat Res Ctr, 1-1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan Natl Inst Adv Ind Sci & Technol 1-1-1Higashi Tsukuba Ibaraki Japan 3058566
Citazione:
N. Ishii et al., "Crystallization and preliminary X-ray studies of V-1-ATPase of Thermus thermophilus HB8 complexed with Mg-ADP", J STRUCT B, 134(1), 2001, pp. 88-92

Abstract

Crystals have been grown of the V-1-ATPase sector of the V-type ATP synthase complex (V0V1) from the thermophilic eubacterium Thermus thermophilus HB8. These crystals are grown by the vapor diffusion method in the presence of 5 mM Mg-ADP, from solutions containing 100 mM sodium acetate and 2 M sodium formate, pH 5.5. The crystals diffracted X rays beyond 3.4 Angstrom in resolution on a synchrotron radiation source. The crystals belong to the trigonal space group P3, with unit cell dimensions of a = b = 89.0 Angstrom, c= 179.2 Angstrom, and gamma = 120 degrees The unit cell presumably contains one molecule of V-1-ATPase and the V-m value is calculated as 3.0 Angstrom (3)/Da. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:29:12