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Titolo:
Proteins and saccharides of the sea urchin organic matrix of mineralization: Characterization and localization in the spine skeleton
Autore:
Ameye, L; De Becker, G; Killian, C; Wilt, F; Kemps, R; Kuypers, S; Dubois, P;
Indirizzi:
Free Univ Brussels, Marine Biol Lab, B-1050 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1050 Lab, B-1050 Brussels, Belgium Free Univ Brussels, IRIBHN, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 BHN, B-1070 Brussels, Belgium Univ Calif Berkeley, Dept Mol Cell Biol, Berkeley, CA 94720 USA Univ CalifBerkeley Berkeley CA USA 94720 ll Biol, Berkeley, CA 94720 USA Vlaasme Instelling Technol Onderzoek, Mat Technol Grp, B-2400 Mol, BelgiumVlaasme Instelling Technol Onderzoek Mol Belgium B-2400 400 Mol, Belgium
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 1, volume: 134, anno: 2001,
pagine: 56 - 66
SICI:
1047-8477(200104)134:1<56:PASOTS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMORPHOUS CALCIUM-CARBONATE; WHEAT-GERM-AGGLUTININ; STRONGYLOCENTROTUS-PURPURATUS; LECTIN HISTOCHEMISTRY; GLYCOPROTEIN GLYCANS; DESIGN STRATEGIES; LARVAL SPICULE; EMBRYO; EXPRESSION; GROWTH;
Keywords:
biomineralization; echinoid; glycoproteins; lectin; organic matrix; saccharide; sialic acid; SM30; SM50; ultrastructural localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Dubois, P Free Univ Brussels, Marine Biol Lab, CP 160-15,50 Ave FD Roosevelt, B-1050Brussels, Belgium Free Univ Brussels CP 160-15,50 Ave FD Roosevelt Brussels Belgium B-1050
Citazione:
L. Ameye et al., "Proteins and saccharides of the sea urchin organic matrix of mineralization: Characterization and localization in the spine skeleton", J STRUCT B, 134(1), 2001, pp. 56-66

Abstract

Properties of the echinoderm skeleton are under biological control, which is exerted in part by the organic matrix embedded in the mineralized part of the skeleton. This organic matrix consists of proteins and glycoproteins whose carbohydrate component is specifically involved in the control mechanisms. The saccharide moiety of the organic matrix of the spines of the echinoid Paracentrotus lividus was characterized using enzyme-linked lectin assays (ELLAs). O-glycoproteins, different types of complex N-glycoproteins, and terminal sialic acids were detected. Sialic acids are known to interact with Ca ions and could play an important role in the mineralization process. Some of the carbohydrate components detected by ELLAs as well as two organic matrix proteins (SM30 and SM50) were localized within different subregions of the spine skeleton using field-emission scanning electron microscopy. The mappings show that some of these components are not homogeneously distributed in the different skeletal subregions. For example, some N-glycoproteins were preferentially located in the putative amorphous subregion of the skeleton, whereas some O-glycoproteins were localized in the subregion where skeletal growth is inhibited. These results suggest that the biologicalcontrol exerted on the skeletal properties can be partly modulated by local differences in the organic matrix composition. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:13:34