Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The applicability of rat and human liver slices to the study of mechanismsof hepatic drug uptake
Autore:
Olinga, P; Hof, IH; Merema, MT; Smit, M; de Jager, MH; Swart, PJ; Slooff, MJH; Meijer, DKF; Groothuis, GMM;
Indirizzi:
Univ Groningen, Inst Drug Explorat, Univ Ctr Pharm, Dept Pharmacokinet & Drug Delivery, NL-9713 AV Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9713 AV V Groningen, Netherlands Univ Hosp, Dept Surg, Div Hepatobiliary Surg & Liver Transplantat, NL-9713EZ Groningen, Netherlands Univ Hosp Groningen Netherlands NL-9713 EZ 9713EZ Groningen, Netherlands
Titolo Testata:
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
fascicolo: 1, volume: 45, anno: 2001,
pagine: 55 - 63
SICI:
1056-8719(200101/02)45:1<55:TAORAH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEPATOCYTES; BILE-ACID; TRANSPORT; SERUM; VIABILITY; ALBUMINS;
Keywords:
modified albumins; rhodamine B; lucigenin; digoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Olinga, P Univ Groningen, Inst Drug Explorat, Univ Ctr Pharm, Dept Pharmacokinet & Drug Delivery, Ant Deusinglaan 1, NL-9713 AV Groningen, Netherlands Univ Groningen Ant Deusinglaan 1 Groningen Netherlands NL-9713 AV
Citazione:
P. Olinga et al., "The applicability of rat and human liver slices to the study of mechanismsof hepatic drug uptake", J PHARM TOX, 45(1), 2001, pp. 55-63

Abstract

In the present study we investigated the applicability of the liver slice model to study mechanisms of drug uptake. Four model compounds were investigated that enter hepatocytes via entirely different membrane transport mechanisms. Rhodamine B (RB), which enters hepatocytes by passive diffusion, was homogeneously distributed throughout the rat liver slice (250 mum thickness) within 5 min, indicating that the penetration rate into the slice and the diffusion rate into the cells are rapid. In contrast, lucigenin (LU), which is taken up by hepatocytes through adsorptive endocytosis, was detectedin the inner cell layers after 15 min. Digoxin uptake into the slice showed a temperature-dependent component and was stereos electively inhibited byquinine, which is compatible with the involvement of a carrier-mediated uptake mechanism. The neo-glycoalbumin Lactose(27)-Human Serum Albumin (Lact(27)-HSA) and the negatively charged Succinylated-Human Serum Albumin (Suc-HSA) entered the slices and were taken up temperature-dependently into hepatocytes and endothelial cells, respectively. The liver slice preparation is a valuable tool to investigate the mechanisms of cellular uptake of drugs. Moreover, the precision-cut liver slices offer the unique possibility to study both hepatocyte and endothelial cell function in human and rat liver. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:38:22