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Titolo:
Experimental localization of Kv1 family voltage-gated K+ channel alpha andbeta subunits in rat hippocampal formation
Autore:
Monaghan, MM; Trimmer, JS; Rhodes, KJ;
Indirizzi:
Wyeth Ayerst Res, Neurosci, Princeton, NJ 08543 USA Wyeth Ayerst Res Princeton NJ USA 08543 Neurosci, Princeton, NJ 08543 USA SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA SUNYStony Brook Stony Brook NY USA 11794 Biol, Stony Brook, NY 11794 USA SUNY Stony Brook, Inst Cell & Dev Biol, Stony Brook, NY 11794 USA SUNY Stony Brook Stony Brook NY USA 11794 Biol, Stony Brook, NY 11794 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 16, volume: 21, anno: 2001,
pagine: 5973 - 5983
SICI:
0270-6474(20010815)21:16<5973:ELOKFV>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTASSIUM CHANNEL; PYRAMIDAL NEURONS; TOPOGRAPHIC ORGANIZATION; ENTORHINAL CORTEX; DENTATE GYRUS; ADULT-RAT; BRAIN; CELLS; DENDROTOXIN; CA1;
Keywords:
long-term potentiation; synaptic plasticity; A-current; dendrotoxin; ibotenic acid; perforant path; mossy fiber; Schaffer collateral; Shaker;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Rhodes, KJ Wyeth Ayerst Res, Neurosci, Princeton, NJ 08543 USA Wyeth Ayerst Res Princeton NJ USA 08543 rinceton, NJ 08543 USA
Citazione:
M.M. Monaghan et al., "Experimental localization of Kv1 family voltage-gated K+ channel alpha andbeta subunits in rat hippocampal formation", J NEUROSC, 21(16), 2001, pp. 5973-5983

Abstract

In the mammalian hippocampal formation, dendrotoxin-sensitive voltage-gated K+ (Kv) channels modulate action potential propagation and neurotransmitter release. To explore the neuroanatomical basis for this modulation, we used in situ hybridization, coimmunoprecipitation, and immunohistochemistry to determine the subcellular localization of the Kv channel subunits Kv1.1, Kv1.2, Kv1.4, and Kv beta2 within the adult rat hippocampus. Although mRNAsencoding all four of these Kv channel subunits are expressed in the cells of origin of each major hippocampal afferent and intrinsic pathway, immunohistochemical staining suggests that the encoded subunits are associated with the axons and terminal fields of these cells. Using an excitotoxin lesionstrategy, we explored the subcellular localization of these subunits in detail. We found that ibotenic acid lesions of the entorhinal cortex eliminated Kv1.1 and Kv1.4 immunoreactivity and dramatically reduced Kv1.2 and Kv beta2 immunoreactivity in the middle third of the dentate molecular layer, indicating that these subunits are located on axons and terminals of entorhinal afferents. Similarly, ibotenic acid lesions of the dentate gyrus eliminated Kv1.1 and Kv1.4 immunoreactivity in the stratum lucidum of CA3, indicating that these subunits are located on mossy fiber axons. Kainic acid lesions of CA3 dramatically reduced Kv1.1 immunoreactivity in the stratum radiatum of CA1-CA3, indicating that Kv1.1 immunoreactivity in these subfields is associated with the axons and terminals of the Schaffer collaterals. Together with the results of coimmunoprecipitation analyses, these data suggestthat action potential propagation and glutamate release at excitatory hippocampal synapses are directly modulated by Kv1 channel complexes predominantly localized on axons and nerve terminals.

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Documento generato il 28/03/20 alle ore 21:48:36