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Titolo:
6-Hydroxydopamine increases the hydroxylation and nitration of phenylalanine in vivo: implication of peroxynitrite formation
Autore:
Ferger, B; Themann, C; Rose, S; Halliwell, B; Jenner, P;
Indirizzi:
Swiss Fed Inst Technol, Behav Neurobiol Lab, CH-8603 Schwerzenbach, Switzerland Swiss Fed Inst Technol Schwerzenbach Switzerland CH-8603 ch, Switzerland Univ London Kings Coll, Guys Kings & St Thomas Sch Biomed Sci, Wolfson CtrAge Related Dis, London WC2R 2LS, England Univ London Kings Coll London England WC2R 2LS London WC2R 2LS, England Univ Marburg, Fac Pharm, Inst Pharmacol & Toxicol, Marburg, Germany Univ Marburg Marburg Germany Inst Pharmacol & Toxicol, Marburg, Germany Univ Marburg, Fac Pharm, Inst Pharmacol & Toxicol, Marburg, Germany Univ Marburg Marburg Germany Inst Pharmacol & Toxicol, Marburg, Germany Natl Univ Singapore, Dept Biochem, Singapore 0511, Singapore Natl Univ Singapore Singapore Singapore 0511 , Singapore 0511, Singapore
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 3, volume: 78, anno: 2001,
pagine: 509 - 514
SICI:
0022-3042(200108)78:3<509:6ITHAN>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREE-RADICAL FORMATION; NITRIC-OXIDE; PARKINSONS-DISEASE; IN-VIVO; AROMATIC HYDROXYLATION; SUPEROXIDE; TYROSINE; DOPAMINE; MPTP; 3-NITROTYROSINE;
Keywords:
6-hydroxydopamine; hydroxyl radicals; microdialysis; Parkinson's disease; peroxynitrite; phenylalanine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ferger, B Swiss Fed Inst Technol, Behav Neurobiol Lab, Schorenstr 16, CH-8603 Schwerzenbach, Switzerland Swiss Fed Inst Technol Schorenstr 16 Schwerzenbach Switzerland CH-8603
Citazione:
B. Ferger et al., "6-Hydroxydopamine increases the hydroxylation and nitration of phenylalanine in vivo: implication of peroxynitrite formation", J NEUROCHEM, 78(3), 2001, pp. 509-514

Abstract

In the present study, we investigated the effect of the dopaminergic neurotoxin 6-hydroxydopamine (6-OHDA) on hydroxyl free radical and peroxynitriteformation in vivo using D-phenylalanine as a novel mechanistic probe. In vivo microdialysis was carried out in the striatum of freely moving male Wistar rats. The microdialysis probes were perfused with artificial cerebrospinal fluid containing 5 MM D-phenylalanine (flow rate 2 muL/min). After obtaining a stable baseline 6-OHDA was delivered into the striatum via reverse microdialysis for 60 min. HPLC measurements of the effluent were performed using photodiode array detection for determination of phenylalanine derivedo-tyrosine and m-tyrosine (as hydroxylation markers) as well as of nitrotyrosine and nitrophenylalanine (as nitration markers). The basal levels of the hydroxylation derived products of phenylalanine were approximately 100-fold higher than those of the nitration derived products. 6-OHDA (0.1, 1, 10mm) significantly increased o- and m-tyrosine up to nine- and 13-fold, respectively, whereas levels of 3-nitrotyrosine and 4-nitrophenylalanine were significantly increased up to 422- and 358-fold, respectively. The results demonstrate that phenylalanine is a sensitive in vivo marker for 6-OHDA-induced hydroxylation and nitration reactions which are clearly concentration dependent. We conclude that peroxynitrite formation is involved in 6-OHDA-induced neurochemical effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:10:29