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Titolo:
Identification and functional characterization of thromboxane A(2) receptors in Schwann cells
Autore:
Muja, N; Blackman, SC; Le Breton, GC; DeVries, GH;
Indirizzi:
Edward Hines Jr Vet Adm Hosp, Res Serv, Hines, IL 60141 USA Edward Hines Jr Vet Adm Hosp Hines IL USA 60141 Serv, Hines, IL 60141 USA Loyola Univ Chicago, Grad Program Neurosci, Maywood, IL USA Loyola Univ Chicago Maywood IL USA rad Program Neurosci, Maywood, IL USA Loyola Univ Chicago, Dept Cell Biol Neurobiol & Anat, Maywood, IL USA Loyola Univ Chicago Maywood IL USA iol Neurobiol & Anat, Maywood, IL USA Univ Illinois, Dept Pharmacol, Chicago, IL USA Univ Illinois Chicago IL USA v Illinois, Dept Pharmacol, Chicago, IL USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 3, volume: 78, anno: 2001,
pagine: 446 - 456
SICI:
0022-3042(200108)78:3<446:IAFCOT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN E-2 RECEPTORS; WALLERIAN DEGENERATION; HUMAN-PLATELETS; CYCLIC-AMP; G-PROTEINS; PROSTANOID BIOSYNTHESIS; MACROPHAGE RESPONSES; PERIPHERAL-NERVE; GROWTH-FACTOR; BETA-ISOFORM;
Keywords:
cAMP; neurofibromatosis type 1; peripheral nerve; Schwann cell; thromboxane A(2); Wallerian degeneration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: DeVries, GH Edward Hines Jr Vet Adm Hosp, Res Serv, Res 151,Bldg 1,Room C423,5th Ave &Roosevelt Rd, Hines, IL 60141 USA Edward Hines Jr Vet Adm Hosp Res 151,Bldg 1,Room C423,5th Ave & Roosevelt Rd Hines IL USA 60141
Citazione:
N. Muja et al., "Identification and functional characterization of thromboxane A(2) receptors in Schwann cells", J NEUROCHEM, 78(3), 2001, pp. 446-456

Abstract

Previous reports have demonstrated the presence of functional thromboxane A(2) (TP) receptors in astrocytes and oligodendrocytes. In these experiments, the presence and function of TP receptors in primary rat Schwann cells (rSC) and a neurofibrosarcoma-derived human Schwann cell line (T265) was investigated. Immunocytochemical and immunoblot analyses using polyclonal anti-TP receptor antibodies demonstrate that both cell types express TP receptors. Treatment with the stable thromboxane A(2) mimetic U46619 (10 muM) did not stimulate intracellular calcium mobilization in rSC, whereas T265 cellsdemonstrated a calcium response that was inhibited by prior treatment withTP receptor antagonists. U46619 also stimulated CREB phosphorylation on Ser133 in T265 cells and, to a lesser extent, in rSC. To identify potential mechanisms of CREB phosphorylation in rSC, we monitored intracellular cAMP levels following U46619 stimulation. Elevated levels of cAMP were detected in both rSC (20-fold) and T265 (15-fold) cells. These results demonstrate that TP receptor activation specifically stimulates CREB phosphorylation in T265 cells, possibly by a calcium and/or cAMP-dependent mechanism. In contrast, TP receptor activation in rSC stimulates increases in cAMP and CREB phosphorylation but does not elicit changes in intracellular calcium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:49:17