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Titolo:
Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic core proteins
Autore:
Okuwaki, M; Iwamatsu, A; Tsujimoto, M; Nagata, K;
Indirizzi:
Univ Tsukuba, Inst Basic Med Sci, Dept Infect Biol, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan RIKEN, Lab Cellular Biochem, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 ar Biochem, Wako, Saitama 3510198, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 1, volume: 311, anno: 2001,
pagine: 41 - 55
SICI:
0022-2836(20010803)311:1<41:IONAAN>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATING FACTOR-I; CHROMATIN-REMODELING COMPLEX; NUCLEOSOME ASSEMBLY PROTEIN; NUCLEAR MATRIX PROTEIN; RNA-POLYMERASE-I; DEPENDENT TRANSCRIPTION; BINDING ACTIVITY; MESSENGER-RNA; TEMPLATE; CELLS;
Keywords:
acidic molecular chaperone; adenovirus; chromatin remodeling; nucleolus; template activating factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Nagata, K Univ Tsukuba, Inst Basic Med Sci, Dept Infect Biol, 1-1-1 Tennohdai, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba 1-1-1 Tennohdai Tsukuba Ibaraki Japan 3058575 Japan
Citazione:
M. Okuwaki et al., "Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic core proteins", J MOL BIOL, 311(1), 2001, pp. 41-55

Abstract

The processes governing chromatin remodeling and assembly, which occur prior to and/or after transcription and replication, are not completely understood. To understand the mechanisms of transcription and replication from chromatin templates, we have established in vitro replication and transcription systems using adenovirus (Ad) DNA complexed with viral basic core proteins, called Ad core, as a template. Using this system, we have previously identified, from HeLa cells, template activating factor-I as a stimulatory factor for the Ad core DNA replication. Here, using this system as a tool, weidentified and purified a novel template activating factor activity that consists of two acidic polypeptides whose apparent molecular masses are 38 kDa and 37 kDa. These two polypeptides correspond to two splicing variants of nucleolar phosphoprotein, nucleophosmin/B23. Recombinant B23 proteins stimulate the Ad core DNA replication, and the acidic regions of B23 proteins are important for its activity. In addition, B23 proteins directly bind to core histones and transfer them to naked DNA. Furthermore, chromatin components such as histories and topoisomerase II are co-immunoprecipitated with B23 from cell extracts. These observations lead to a hypothesis that nucleophosmin/B23 is involved in structural changes of chromatin, thereby regulating transcription and replication within the ribosomal DNA region or maintaining the nucleolar structure. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:13:07