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Titolo:
Reaction monitoring of platinum(II) complex-5 '-guanosine monophosphate adduct formation by ion exchange liquid chromatography/electrospray ionization mass spectrometry
Autore:
Zollner, P; Zenker, A; Galanski, M; Keppler, BK; Lindner, W;
Indirizzi:
Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria Univ Vienna ViennaAustria A-1090 st Analyt Chem, A-1090 Vienna, Austria Univ Vienna, Inst Inorgan Chem, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 t Inorgan Chem, A-1090 Vienna, Austria
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 7, volume: 36, anno: 2001,
pagine: 742 - 753
SICI:
1076-5174(200107)36:7<742:RMOPC'>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DRUG-DNA ADDUCTS; CAPILLARY-ELECTROPHORESIS; COMPLEXES; CISPLATIN; OLIGONUCLEOTIDES; SEPARATION; NUCLEOTIDE; BINDING; CIS-(2+); SUBSTITUTION;
Keywords:
platinum(II) complex; guanosine monophosphate; ion-exchange chromatography; electrospray ionization mass spectrometry; liquid chromatography/mass spectrometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Zollner, P Univ Vienna, Inst Analyt Chem, Wahringerstr 38, A-1090 Vienna, Austria Univ Vienna Wahringerstr 38 Vienna Austria A-1090 nna, Austria
Citazione:
P. Zollner et al., "Reaction monitoring of platinum(II) complex-5 '-guanosine monophosphate adduct formation by ion exchange liquid chromatography/electrospray ionization mass spectrometry", J MASS SPEC, 36(7), 2001, pp. 742-753

Abstract

Cisplatin and four structurally related platinum(II) complexes were incubated with guanosine 5 ' -monophosphate (5 ' -GMP) in water at 37 degreesC. The adduct formation reactions were monitored with cation-and anion-exchangeliquid chromatography/electrospray ionization mass spectrometry. In addition to mono- and bis-adducts of guariosine 5 ' -monophosphate with the platinum(II) complexes, other molecular species, presumably with a binuclear structure (two platinum(II) centres), were detected in the reaction mixtures, which have not been reported previously, indicating an unexpected complexity of adduct formation. Anion-exchange chromatography revealed the presence of isomers of two complexes which presumably result from the restricted rotation at the platinum-N-7 (5 ' -GMP) bonds. All reaction products were characterized in both the positive and negative ion modes. Furthermore, preliminary kinetics and half-times of complex formation were investigated for cisplatin and two other platinum(fl) complexes, monitoring the relative concentrations of free 5 ' -GMP and of mono- and bis-GMP adducts as a function oftime (250 h) using an internal standard protocol with thymidine 5 ' -monophosphate. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 02/04/20 alle ore 02:09:15