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Titolo:
Determination of famotidine in low-volume human plasma by normal-phase liquid chromatography/tandem mass spectrometry
Autore:
Zhong, L; Eisenhandler, R; Yeh, KC;
Indirizzi:
Merck Res Labs, Dept Drug Metab, W Point, PA 19486 USA Merck Res Labs W Point PA USA 19486 ept Drug Metab, W Point, PA 19486 USA
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 7, volume: 36, anno: 2001,
pagine: 736 - 741
SICI:
1076-5174(200107)36:7<736:DOFILH>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-SECRETION; PHARMACOKINETICS; PHARMACODYNAMICS; QUANTIFICATION; SUPPRESSION; CHILDREN;
Keywords:
famotidine; infants; pharmacokinetics; liquid chromatography/tandem mass spectrometry; biological fluid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Zhong, L Merck Res Labs, Dept Drug Metab, WP75A-303, W Point, PA 19486 USAMerck Res Labs WP75A-303 W Point PA USA 19486 Point, PA 19486 USA
Citazione:
L. Zhong et al., "Determination of famotidine in low-volume human plasma by normal-phase liquid chromatography/tandem mass spectrometry", J MASS SPEC, 36(7), 2001, pp. 736-741

Abstract

A rapid, sensitive and robust assay procedure using liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) for the determination of famotidine in human plasma and urine is described. Famotidine and the internal standard were isolated from plasma samples by cation-exchange solid-phase extraction with benzenesulfonic acid (SCX) cartridges. The urine assay used direct injection of a diluted urine sample. The chromatographic separation was accomplished by using a BDS Hypersil silica column with a mobile phase of acetonitrile-water containing trifluoroacetic acid. The MS/MS detection of the analytes was set in the positive ionization mode using electrospray ionization for sample introduction. The analyte and internal standard precursor-product ion combinations were monitored in the multiple-reaction monitoring mode. Assay calibration curves were linear in the concentration range 0.5-500 ng ml(-1) and 0.05-50 pg ml(-1) in plasma and urine, respectively. For the plasma assay, a 100 mul sample aliquot was subjected to extraction. To perform the urine assay, a 50 mul sample aliquot was used. The intra-day relative standard deviations at all concentration levels were < 10%. The inter-day consistency was assessed by running quality control samples during each daily run. The limit of quantification was 0.5 ng ml(-1) in plasma and 0.05 mug ml(-1) in urine. The methods were utilized to support clinical pharmacokinetic studies in infants aged 0-12 months. Copyright (C) 2001John Wiley & Sons, Ltd.

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Documento generato il 19/01/20 alle ore 20:26:02