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Titolo:
Use of L-[N-15] glutamic acid and homoglutathione to determine both glutathione synthesis and concentration by gas chromatography-mass spectrometry (GCMS)
Autore:
Humbert, B; Nguyen, P; Obled, C; Bobin, C; Vaslin, A; Sweeten, S; Darmaun, D;
Indirizzi:
Nemours Childrens Clin, Jacksonville, FL USA Nemours Childrens Clin Jacksonville FL USA ns Clin, Jacksonville, FL USA Inst Natl Rech Agron, Theix, France Inst Natl Rech Agron Theix FranceInst Natl Rech Agron, Theix, France Ecole Natl Vet, Unite Nutr & Alimentat, Nantes, France Ecole Natl Vet Nantes France et, Unite Nutr & Alimentat, Nantes, France Ctr Rech Nutr Humaine, INSERM U539, Nantes, France Ctr Rech Nutr Humaine Nantes France umaine, INSERM U539, Nantes, France
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 7, volume: 36, anno: 2001,
pagine: 726 - 735
SICI:
1076-5174(200107)36:7<726:UOLGAA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEFICIENCY; INFECTION;
Keywords:
stable isotopes; glutathione; glutamate; erythrocyte; dog;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Darmaun, D Hop Hotel Dieu, INSERM U539, HNB 3e Etage Nord, F-44093 Nantes 1, France Hop Hotel Dieu HNB 3e Etage Nord Nantes France 1 tes 1, France
Citazione:
B. Humbert et al., "Use of L-[N-15] glutamic acid and homoglutathione to determine both glutathione synthesis and concentration by gas chromatography-mass spectrometry (GCMS)", J MASS SPEC, 36(7), 2001, pp. 726-735

Abstract

A method for simultaneous measurement of both glutathione enrichment and concentration in a biological sample using gas chromatography mass spectrometry is described. The method is based on the preparation of N,S-ethoxycarbonylmethyl ester derivatives of glutathione, and the use of homoglulathione (glutamyl-cysteinyl-alanine) as an internal standard. A procedure for determination of glutamate concentration and enrichment is also reported. Both methods have within-day and day-to-day interassay coefficients of variation less than 5%, and recoveries of known added amounts of glutathione and glutamate are close to 100%. Taken together, these methods allowed determination of glutathione concentration and fractional synthesis rate in red blood cells Using L-[N-15] glutamic acid infusion. This approach was applied in vivo to investigate the effects of a 72 h fast, compared with a control overnight fast, on erythrocyte glutathione in a single dog. The 72 h fast was associated with a 39% decline in erythrocyte glutathione level, (2.9 +/- 0.4 versus 4.7 +/- 0.5 mmol l(-1), fasting versus control) with no change in glutathione fractional synthesis (67.4 versus 71.3% d(-1), fasting versus control). Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:14:18