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Titolo:
Immunochemical detection of a lipofuscin-like fluorophore derived from malondialdehyde and lysine
Autore:
Yamada, S; Kumazawa, S; Ishii, T; Nakayama, T; Itakura, K; Shibata, N; Kobayashi, M; Sakai, K; Osawa, T; Uchida, K;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Lab Food & Biodynam, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 ynam, Nagoya, Aichi 4648601, Japan NOF Co, Tsukuba Res Lab, Tsukuba, Ibaraki 3002635, Japan NOF Co Tsukuba Ibaraki Japan 3002635 Lab, Tsukuba, Ibaraki 3002635, Japan Univ Shizouka, Dept Food & Nutr Sci, Shizuoka 4228529, Japan Univ Shizouka Shizuoka Japan 4228529 & Nutr Sci, Shizuoka 4228529, Japan Aichi Univ Educ, Fac Educ, Kariya, Aichi 448, Japan Aichi Univ Educ Kariya Aichi Japan 448 Fac Educ, Kariya, Aichi 448, Japan Tokyo Womens Med Univ, Dept Pathol, Tokyo 1628666, Japan Tokyo Womens Med Univ Tokyo Japan 1628666 t Pathol, Tokyo 1628666, Japan
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 8, volume: 42, anno: 2001,
pagine: 1187 - 1196
SICI:
0022-2275(200108)42:8<1187:IDOALF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; PEROXIDIZED LIPIDS; OXIDATIVE MODIFICATION; APOLIPOPROTEIN-B; AMINO-ACIDS; MALONALDEHYDE; ANTIOXIDANTS; PRODUCTS; RABBITS;
Keywords:
lipid peroxidation; protein modification; dihydropyridine; monoclonal antibody;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Uchida, K Nagoya Univ, Grad Sch Bioagr Sci, Lab Food & Biodynam, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 ya, Aichi 4648601, Japan
Citazione:
S. Yamada et al., "Immunochemical detection of a lipofuscin-like fluorophore derived from malondialdehyde and lysine", J LIPID RES, 42(8), 2001, pp. 1187-1196

Abstract

The accumulation of fluorescent age pigment or lipofuscin is a frequently observed age-associated cellular alteration in a variety of postmitotic cells of many species. These pigments are observed within granules composed, in part, of damaged protein and lipid. Modification of various biomolecules by aldehyde products of lipid peroxidation is believed to contribute to lipofuscin and ceroid formation. In the present study, we raised a monoclonal antibody (MAb 1F83) directed to the malondialdehyde-modified protein and identified a lipofuscin-like dihydropyridine fluorophore as the major epitope. This antibody was used to conclusively demonstrate that the fluorophore forms on oxidatively modified low density lipoproteins. In addition, we demonstrated that the materials immunoreactive to MAb 1F83 indeed constituted the atherosclerotic lesions, in which intense positivity was associated primarily with macrophage-derived foam cells.ie The results of this study suggest that the reaction between the lipid peroxidation-derived aldehyde and primary amino groups of protein might represent a process common to the formation of the lipofuscin-like fluorophore during aging and its related diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 03:00:53