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Titolo:
Augmented TNF-alpha and IL-10 production by primed human monocytes following interaction with oxidatively modified autologous erythrocytes
Autore:
Liese, AM; Siddiqi, MQ; Siegel, JH; Denny, T; Spolarics, Z;
Indirizzi:
Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Anat, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 Anat, Newark, NJ 07103 USA Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Cell Biol & Injury Sci, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 Sci, Newark, NJ 07103 USA Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Pediat, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 diat, Newark, NJ 07103 USA
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 2, volume: 70, anno: 2001,
pagine: 289 - 296
SICI:
0741-5400(200108)70:2<289:ATAIPB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-CELL DEFORMABILITY; NECROSIS-FACTOR-ALPHA; CYTOKINE PRODUCTION; PROINFLAMMATORY CYTOKINES; PHAGOCYTIC FUNCTION; FLOW-CYTOMETRY; WHOLE-BLOOD; SEPSIS; PROTEIN; LIPOPOLYSACCHARIDE;
Keywords:
red blood cells; cytokines; oxidative stress; macrophage; host response; inflammation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Spolarics, Z Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Anat, 185 S Orange Ave,MSB G-626, Newark, NJ 07103 USA Univ Med & Dent New Jersey 185 S Orange Ave,MSB G-626 Newark NJ USA 07103
Citazione:
A.M. Liese et al., "Augmented TNF-alpha and IL-10 production by primed human monocytes following interaction with oxidatively modified autologous erythrocytes", J LEUK BIOL, 70(2), 2001, pp. 289-296

Abstract

The presence of dysfunctional/damaged red blood cells (RBCs) has been associated with adverse clinical effects during the inflammatory response. The aim of this study was to elucidate whether oxidatively modified, autologous, RBCs modulate monocyte cytokine responses in humans. Monocyte tumor necrosis factor alpha (TNF-alpha) and IL-10 production was measured in whole blood from healthy volunteers using ELISA and flow cytometry. Oxidatively modified RBCs (15 m.M phenylhydrazine, 1 h, OX-RBQ or vehicle-treated RBCs (VT-RBC) opsonized by autologous serum were administered alone or in combination with one of three priming agents: E. coli lipopolysaccharide (LPS, 0.2 ng/ml), zymosan A (1 mg/ml), or phorbol 12-myristate 13-acetate (PMA, 50 ng/ml). OX-RBC or W-RBC alone did not result in the release of TNF-alpha or IL-10. LPS, zymosan, and PMA caused marked and dose-dependent increases in TNF-alpha and IL-10 production. Addition of OX-RBC augmented the LPS-, zymosan-, and PMA-induced TNF-alpha release by approximately 100%. OX-RBC augmented LPS- and zymosan-mduced IL-10 release by 400-600%. Flow cytometry analyses showed that monocytes were responsible for TNF-alpha and IL-10 productionin whole blood. The presence of OX-RBC alone increased the complexity of CD14+ monocytes but caused no cytokine production. LPS alone induced cytokine production without altering cell complexity. After the combined (OX-RBC+LPS) treatment, monocytes of high complexity were responsible for TNF-alpha production. The presence of mannose or galactose (at 10-50 mM) did not alter the observed augmentation of cytokine production by OX-RBC, suggesting that lectin receptors are not involved in the response. These studies indicate that the interaction between damaged autologous erythrocytes and monocytes has a major impact on the cytokine responses in human. An augmented cytokine production by the mononuclear phagocyte system may adversely affect theclinical course of injury and infections especially in genetic or acquiredRBC diseases or after transfusions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 01:05:23