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Titolo:
Voltage-dependent and calcium-activated ion channels in the human mast cell line HMC-1
Autore:
Duffy, SM; Leyland, ML; Conley, EC; Bradding, P;
Indirizzi:
Univ Leicester, Sch Med, Inst Lung Hlth, Div Resp Med, Leicester LE1 7RH, Leics, England Univ Leicester Leicester Leics England LE1 7RH er LE1 7RH, Leics, England
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 2, volume: 70, anno: 2001,
pagine: 233 - 240
SICI:
0741-5400(200108)70:2<233:VACICI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLORIDE CHANNEL; HUMAN LUNG; MOLECULAR CHARACTERIZATION; DENTS-DISEASE; K+-CHANNELS; PATCH-CLAMP; CL CHANNEL; EXPRESSION; CLONING; KIDNEY;
Keywords:
human; mast cells; chloride; potassium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Bradding, P Glenfield Gen Hosp, Dept Resp Med, Groby Rd, Leicester LE3 9QP, Leics, England Glenfield Gen Hosp Groby Rd Leicester Leics England LE3 9QP nd
Citazione:
S.M. Duffy et al., "Voltage-dependent and calcium-activated ion channels in the human mast cell line HMC-1", J LEUK BIOL, 70(2), 2001, pp. 233-240

Abstract

The mechanisms underlying the recruitment, differentiation, and sustained activation of mast cells in disease are likely to include modulation of ionchannels. Specific Ca2+, K+, and Cl- conductances have been identified in rodent mast cells, but there are no equivalent data on human mast cells. Wehave used the whole-cell patch-clamp technique to characterize macroscopicion currents in both the human mast cell line HMC-1 and human skin mast cells (HSMCs) at rest and in HMC-1 after activation with calcium ionophore. HSMCs were electrically silent at rest. In contrast, HMC-1 expressed a strong outwardly rectifying voltage-dependent Cl- conductance characteristic of ClC-4 or ClC-5 and a small inwardly rectifying K+ current not carried by the classical Kir family of K+ channels. Calcium ionophore induced the appearance of outwardly rectifying Ca2+-activated Cl- and K+ currents, while hypotonicity induced another outwardly rectifying conductance typical of ClC-3. Reverse transcription-PCRs confirmed that mRNAs for the voltage-dependent Cl- channels ClC-3 and -5 were expressed. This is the first definitive description of a ClC-4/5-like current in a native leukocyte. We suggest that this current may contribute to the malignant phenotype while the Ca2+-activated K+ and Cl- currents may he involved in cell activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:08:46