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Titolo:
Simultaneous determination of six adenyl purines in human plasma by high-performance liquid chromatography with fluorescence derivatization
Autore:
Katayama, M; Matsuda, Y; Shimokawa, K; Tanabe, S; Kaneko, S; Hara, I; Sato, H;
Indirizzi:
Meiji Pharmaceut Univ, Dept Funct Bioanal, Kiyose, Tokyo 2048588, Japan Meiji Pharmaceut Univ Kiyose Tokyo Japan 2048588 se, Tokyo 2048588, Japan Ichikawa Gen Hosp, Tokyo Dent Coll, Dept Obstet & Gynecol, Chiba 2728513, Japan Ichikawa Gen Hosp Chiba Japan 2728513 et & Gynecol, Chiba 2728513, Japan Tachikawa Kyosai Hosp, Dept Obstet & Gynecol, Tokyo, Japan Tachikawa Kyosai Hosp Tokyo Japan , Dept Obstet & Gynecol, Tokyo, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 760, anno: 2001,
pagine: 159 - 163
SICI:
1387-2273(20010825)760:1<159:SDOSAP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENINE-NUCLEOTIDES; DENSITY GRADIENT; ADENOSINE; SPERM;
Keywords:
adenyl purines; adenine; adenosine; AMP; ADP; ATP; cyclic AMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Katayama, M Meiji Pharmaceut Univ, Dept Funct Bioanal, 2-522-1 Noshio, Kiyose, Tokyo 2048588, Japan Meiji Pharmaceut Univ 2-522-1 Noshio Kiyose TokyoJapan 2048588
Citazione:
M. Katayama et al., "Simultaneous determination of six adenyl purines in human plasma by high-performance liquid chromatography with fluorescence derivatization", J CHROMAT B, 760(1), 2001, pp. 159-163

Abstract

A sensitive method was developed for the simultaneous determination of sixadenyl purines in human plasma by high-performance liquid chromatography. The adenyl purines (adenine, adenosine, AMP, ADP, ATP and cyclic AMP) were derivatized using 2-chloroacetaldehyde for fluorescence detection, and the reaction and separation conditions were reinvestigated to improve sensitivity for small volume sample analysis. Each derivatized purine was separated on a Capcell Pack SG120A(TM) column with mobile phase consisting of 0.05 M citric acid-0.1 M dipotassium hydrogen phosphate (pH 4.0)-methanol (97+3). The detection limits were 100-1000 fmol/ml by fluorescence detection, some 500 times better than previous reports. The proposed method was applied to determine adenyl purines in human plasma. The purine levels were as follows: ATP (9.2-22.2 pmol/ml), ADP (5.5-22.2 pmol/ml), AMP (0.8-3.2 pmol/ml). Other purines, adenine, adenosine, cAMP were lower than 0.1 pmol/ml. (C) 2001Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:55:46