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Titolo:
Polyhydroxyethylmethacrylate-based magnetic DNA-affinity beads for anti-DNA antibody removal from systemic lupus erythematosus patient plasma
Autore:
Odabasi, M; Denizli, A;
Indirizzi:
Univ Hacettepe, Dept Chem, Div Biochem, TR-06100 Ankara, Turkey Univ Hacettepe Ankara Turkey TR-06100 v Biochem, TR-06100 Ankara, Turkey
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 760, anno: 2001,
pagine: 137 - 148
SICI:
1387-2273(20010825)760:1<137:PMDBFA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
STABILIZED FLUIDIZED-BED; HUMAN-IMMUNOGLOBULIN-G; PHOSPHORYLATED POLYSTYRENE; MICROSPHERES; MICROBEADS; IMMOBILIZATION; CHROMATOGRAPHY; ADSORPTION; SORPTION; BINDING;
Keywords:
affinity beads; DNA; polyhydroxyethylmethacrylate; anti-DNA antibody; HIgG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Denizli, A PK 51, TR-06242 Ankara, Turkey PK 51 Ankara Turkey TR-06242PK 51, TR-06242 Ankara, Turkey
Citazione:
M. Odabasi e A. Denizli, "Polyhydroxyethylmethacrylate-based magnetic DNA-affinity beads for anti-DNA antibody removal from systemic lupus erythematosus patient plasma", J CHROMAT B, 760(1), 2001, pp. 137-148

Abstract

The aim of this study is to prepare magnetic poly(2-hydroxyethylmethacrylate) (mPHEMA) beads and to investigate their utility for the removal of anti-DNA antibodies from systemic lupus erythematosus (SLE) patient plasma. mPHEMA beads, in the size range of 80-120 mum, were produced by a modified suspension technique. Then, DNA was coupled onto mPHEMA beads by carbodiimide activation. The amount off ligand coupled was changed by changing the initial concentrations of carbodiimide and DNA. Human immunoglobulin G (HIgG) and anti-DNA antibody adsorption from aqueous solutions and human plasma wereexamined in a batch system. mPHEMA beads were characterized by swelling tests, electron spin resonance (ESR) and scanning electron microscopy. Important results obtained in this study are as follows: the swelling ratio of mPHEMA beads was 34%. The presence of magnetite particles in the polymeric structure was confirmed by ESR. The mPHEMA beads have a spherical shape and porous structure. Maximum DNA coupling of carbodiimide activated mPHEMA beads was 4.4 mg/g. Maximum HIgG adsorption from an aqueous solution was 47.5 mg/g. Anti-DNA antibody adsorption from SLE plasma was observed as 87.6 mg/g. Non-specific HIgG adsorption was 0.1 mg/g. More than 90% of the adsorbed HIgG molecules and anti-DNA antibodies were desorbed succesfully by using NaSCN solution. It was possible to reuse these DNA-affinity beads without significant losses in the antibody adsorption capacities. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 23:02:19