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Titolo:
Immobilised metal-ion affinity chromatography purification of histidine-tagged recombinant proteins: a wash step with a low concentration of EDTA
Autore:
Westra, DF; Welling, GW; Koedijk, DGAM; Scheffer, AJ; The, TH; Welling-Wester, S;
Indirizzi:
Univ Groningen, Dept Med Microbiol, NL-9713 GZ Groningen, Netherlands UnivGroningen Groningen Netherlands NL-9713 GZ Z Groningen, Netherlands Univ Groningen, Dept Clin Immunol, NL-9713 GZ Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9713 GZ Z Groningen, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 760, anno: 2001,
pagine: 129 - 136
SICI:
1387-2273(20010825)760:1<129:IMACPO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTAINING CYTOCHROME-C; VIRUS; GLYCOPROTEIN; GH; COMPLEX; BINDING; GL;
Keywords:
recombinant proteins; EDTA; histidine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Welling-Wester, S Univ Groningen, Dept Med Microbiol, Hanzepl 1, NL-9713 GZ Groningen, Netherlands Univ Groningen Hanzepl 1 Groningen Netherlands NL-9713 GZ
Citazione:
D.F. Westra et al., "Immobilised metal-ion affinity chromatography purification of histidine-tagged recombinant proteins: a wash step with a low concentration of EDTA", J CHROMAT B, 760(1), 2001, pp. 129-136

Abstract

Immobilised metal-ion affinity chromatography (IMAC) is widely used for the purification of recombinant proteins in which a poly-histidine tag is introduced. However, other proteins may also bind to IMAC columns. We describethe use of a washing buffer with a low concentration of EDTA (0.5 mM) for the removal of proteins without histidine tag from IMAC columns. Four histidine-tagged recombinant proteins/protein complexes were purified to homogeneity from cell culture medium of insect cells by using an EDTA washing buffer. The presence of a low concentration of EDTA in washing buffers during IMAC may have a general application in the purification of histidine-tagged proteins. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:15:44