Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Simultaneous determination of unbound ropivacaine in rat blood and brain using microdialysis
Autore:
Kau, YC; Wong, KM; Shyr, MH; Lee, YH; Tsai, TH;
Indirizzi:
Natl Res Inst Chinese Med, Taipei 112, Taiwan Natl Res Inst Chinese Med Taipei Taiwan 112 nese Med, Taipei 112, Taiwan Chang Gung Mem Hosp, Lin Kou Med Ctr, Dept Anesthesiol, Tao Yuan 111, Taiwan Chang Gung Mem Hosp Tao Yuan Taiwan 111 nesthesiol, Tao Yuan 111, Taiwan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 760, anno: 2001,
pagine: 107 - 112
SICI:
1387-2273(20010825)760:1<107:SDOURI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEADY-STATE VOLUME; LIQUID-CHROMATOGRAPHY; BUPIVACAINE; PLASMA; PHARMACOKINETICS; METABOLITES; VALIDATION; TRANSPORT;
Keywords:
ropivacaine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Tsai, TH Natl Res Inst Chinese Med, 155-1 Li Nong St Sect 2, Taipei 112, Taiwan Natl Res Inst Chinese Med 155-1 Li Nong St Sect 2 Taipei Taiwan 112
Citazione:
Y.C. Kau et al., "Simultaneous determination of unbound ropivacaine in rat blood and brain using microdialysis", J CHROMAT B, 760(1), 2001, pp. 107-112

Abstract

To investigate the pharmacokinetics of ropivacaine in rat blood and brain,a sensitive HPLC method and microdialysis were developed for the simultaneous determination of unbound ropivacaine in rat blood and brain. Adult, male Sprague-Dawley rats (290-350 g) were anesthetized with sodium pentobarbital (50 mg/kg, i.p.). Two microdialysis probes were inserted, one into the jugular vein toward right atrium, and one into the brain striatum of rats. Ropivacaine (5 mg/kg, i.v.) was then administered via the femoral vein. Blood and brain dialysates were collected and eluted with a mobile phase containing methanol-acetonitrite-20 mM monosodium phosphoric acid (pH 5.5) (10:40:50, v/v/v) in a liquid chromatographic system. Separation of ropivacaine was achieved by a CN column (Phenomenex Luna, 250x4.6 mm, particle size 5 mum; Torrance, CA, USA) within 10 min. The UV detector wavelength was set at 205 nm and the detection limit of ropivacaine was 20 ng/ml. The intra- and inter-day accuracy and precision of the analyses were less than 10% in the ranges of 0.02-5 mug/ml. The pharmacokinetic data were calculated from the individual animal measurements of dialysate concentration versus time. Thismethod exhibits no endogenous interference and its sensitivity is sufficient for the determination of biological samples. The present results confirmthat microdialysis sampling followed by LC separation with UV detection represents a viable approach for the measurement of free ropivacaine in rat brain and plasma. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:03:14