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Titolo:
Direct determination by high-performance liquid chromatography of sn-2 monopalmitin after enzymatic lipase hydrolysis
Autore:
Lopez-Lopez, A; Castellote-Bargallo, AI; Lopez-Sabater, MC;
Indirizzi:
Univ Barcelona, Fac Farm, CeRTA, Dept Nutr & Bromatol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 romatol, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 760, anno: 2001,
pagine: 97 - 105
SICI:
1387-2273(20010825)760:1<97:DDBHLC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT-SCATTERING DETECTION; HUMAN-MILK; FATTY-ACIDS; TRIGLYCERIDE COMPOSITION; GAS-CHROMATOGRAPHY; PALMITIC ACID; TRIACYLGLYCEROLS; INFANTS; FORMULAS; HPLC;
Keywords:
sn-2 monopalmitin; pancreatic lipase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Lopez-Sabater, MC Univ Barcelona, Fac Farm, CeRTA, Dept Nutr & Bromatol, Avda Joan XXIII S-N, E-08028 Barcelona, Spain Univ Barcelona Avda Joan XXIIIS-N Barcelona Spain E-08028
Citazione:
A. Lopez-Lopez et al., "Direct determination by high-performance liquid chromatography of sn-2 monopalmitin after enzymatic lipase hydrolysis", J CHROMAT B, 760(1), 2001, pp. 97-105

Abstract

An alternative method to determine the sn-2 monopalmitin in infant formulas was developed and validated. This method offers many advantages over the traditional methods. It follows the official method in the first steps, purification of the fat or oil through an alumina column, and subsequently thetriglycerides are incubated with pancreatic lipase in order to obtain the sn-2 monoglycerides. In traditional methods the sn-2 monoglycerides are separated by preparative thin-layer chromatography and then, the 2-monoglycerides are converted into the corresponding fatty acid methyl esters and analysed by gas chromatography. In our method, separation, quantification and identification of the sn-2 monoglycerides were achieved by high-performance liquid chromatography with evaporative light-scattering detection. The detection limit (0.19 mug), quantification limit (0.38 mug), linearity range (r=0.999, range 1-200 mug) and precision (SD=1.10) show the suitability of theproposed method. This method is faster, cheaper and simple and does not consume large quantities of reagents and materials. (C) 2001 Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:08:47