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Titolo:
Determination of N-acetylation phenotype using caffeine as a metabolic probe and high-performance liquid chromatography with either ultraviolet detection or electrospray mass spectrometry
Autore:
Baud-Camus, F; Marquet, P; Soursac, M; Davrinche, C; Farinotti, R;
Indirizzi:
Fac Pharm Chatenay Malabry, UPRES 2706, Dept Clin Pharm, F-92296 Chatenay Malabry, France Fac Pharm Chatenay Malabry Chatenay Malabry France F-92296 labry, France Hop Univ Dupuytren, Serv Pharmacol & Toxicol, F-87042 Limoges, France Hop Univ Dupuytren Limoges France F-87042 xicol, F-87042 Limoges, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 760, anno: 2001,
pagine: 55 - 63
SICI:
1387-2273(20010825)760:1<55:DONPUC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY ELECTROPHORESIS; URINARY METABOLITES; NAT2 PHENOTYPES; ACETYLTRANSFERASE;
Keywords:
N-acetylation phenotype; caffeine; 1-methylxanthine; 1-methyluric acid; 5-acetylamino-6-amino-3-methyluracil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Baud-Camus, F Fac Pharm Chatenay Malabry, UPRES 2706, Dept Clin Pharm, 5 Rue Jean Baptiste Clement, F-92296 Chatenay Malabry, France Fac Pharm Chatenay Malabry 5 Rue Jean Baptiste Clement Chatenay Malabry France F-92296
Citazione:
F. Baud-Camus et al., "Determination of N-acetylation phenotype using caffeine as a metabolic probe and high-performance liquid chromatography with either ultraviolet detection or electrospray mass spectrometry", J CHROMAT B, 760(1), 2001, pp. 55-63

Abstract

A rapid, sensitive method using liquid chromatography-electrospray mass spectrometry (LC-ES-MS) was developed and evaluated for the simultaneous quantitative determination of caffeine metabolites 1U, 1X and AAMU in human urine. This method involved a simple dilution of urine samples. The chromatographic separation was achieved on a C-18 reversed-phase column using a gradient of acetonitrile in 2 mM, pH 3.0 ammonium formate as mobile phase. Afterionisation in an electrospray source, mass spectrometric detection was performed in the negative ion, selected ion monitoring mode. This method yielded acceptable accuracy and precision within the range 0.25-50 mug/ml. This analytical method was applied to investigate the N-acetylator phenotype of HIV infected patients and compared with high-performance liquid chromatography with UV detection. Its specificity was better, which appeared to be absolutely necessary to prevent errors in metabolic ratios and phenotype interpretation. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:59:28