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Titolo:
Mutation in PMR1, a Ca2+-ATPase in Golgi, confers salt tolerance in Saccharomyces cerevisiae by inducing expression of PMR2, an Na+-ATPase in plasma membrane
Autore:
Park, SY; Seo, SB; Lee, SJ; Na, JG; Kim, YJ;
Indirizzi:
Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea Pusan Natl UnivPusan South Korea 609735 Biol, Pusan 609735, South Korea Kunsan Natl Univ, Sch Sci & Technol, Kunsan 573701, South Korea Kunsan Natl Univ Kunsan South Korea 573701 l, Kunsan 573701, South Korea
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 31, volume: 276, anno: 2001,
pagine: 28694 - 28699
SICI:
0021-9258(20010803)276:31<28694:MIPACI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATASE CALCINEURIN; TRANSCRIPTION FACTOR; SECRETORY PATHWAY; H+/CA2+ EXCHANGE; GENE-EXPRESSION; NACL TOLERANCE; CA2+ ATPASES; YEAST-CELLS; STRESS; TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, YJ Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea Pusan Natl Univ Pusan South Korea 609735 san 609735, South Korea
Citazione:
S.Y. Park et al., "Mutation in PMR1, a Ca2+-ATPase in Golgi, confers salt tolerance in Saccharomyces cerevisiae by inducing expression of PMR2, an Na+-ATPase in plasma membrane", J BIOL CHEM, 276(31), 2001, pp. 28694-28699

Abstract

Sodium tolerance in yeast is enhanced by continuous activation of calcineurin, a Ca2+/calmodulin-dependent protein phosphatase that is required for modulation of the Na+ efflux mechanism. We isolated several salt-tolerant mutations with the treatment of ethylmethane sulfonate under high salt stress. One of the mutations was mapped in the PMR1 gene. Pmr1p, the P-type Ca2+-ATPase in the Golgi apparatus, regulates a cytosolic Ca2+ level in variousresponses. Cytosolic Ca2+ concentration in the pmr1 mutant is highly maintained, and thus calcineurin is activated continuously. The treatment of FK506, a specific inhibitor of calcineurin, abolishes the salt-tolerant phenotype of the pmr1 mutant. Activated calcineurin induces the expression of PMR2, encoding the P-type Na+-ATPase, through the specific transcription factor, Tcn1p/Crz1p. Also, expression of the PMR2::lacZ reporter gene in the pmr1 mutant was higher than that in wild type. We propose that the pmr1 mutation confers salt tolerance through continuous activation of calcineurin and that Pmr1p might act as a major Ca2+-ATPase under high salt stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:47:52