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Titolo:
Na+-dependent pH regulation by the amitochondriate protozoan parasite Giardia intestinalis
Autore:
Biagini, GA; Knodler, LA; Saliba, KJ; Kirk, K; Edwards, MR;
Indirizzi:
Univ New S Wales, Sch Biochem & Mol Genet, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 t, Sydney, NSW 2052, Australia Australian Natl Univ, Fac Sci, Sch Biochem & Mol Biol, Canberra, ACT 0200,Australia Australian Natl Univ Canberra ACT Australia 0200 erra, ACT 0200,Australia
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 31, volume: 276, anno: 2001,
pagine: 29157 - 29162
SICI:
0021-9258(20010803)276:31<29157:NPRBTA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; H+-ATPASE; INTRACELLULAR PH; PLASMA-MEMBRANE; CYTOPLASMIC PH; GASTROINTESTINAL PH; LEISHMANIA-DONOVANI; CRITHIDIA-LUCILIAE; TRYPANOSOMA-CRUZI; TRANSIT TIMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Biagini, GA Univ Cambridge, Dept Pharmacol, Tennis Court Rd, Cambridge CB21QJ, England Univ Cambridge Tennis Court Rd Cambridge England CB2 1QJ land
Citazione:
G.A. Biagini et al., "Na+-dependent pH regulation by the amitochondriate protozoan parasite Giardia intestinalis", J BIOL CHEM, 276(31), 2001, pp. 29157-29162

Abstract

Giardia intestinalis is a pathogenic fermentative parasite, which inhabitsthe gastrointestinal tract of animals and humans. G. intestinalis trophozoites are exposed to acidic fluctuations in vivo and must also cope with acidic metabolic endproducts. In this study, a combination of independent techniques (UP NMR spectroscopy, distribution of the weak acid pH marker 5,5-dimethyl-2,4-oxazolidinedione (DMO) and the fluorescent pH indicator 2 ' ,7 '-bis (carboxyethyl)-5,6-carboxyfluorescein (BCECF)) were used to show thatG. intestinalis trophozoites exposed to an extracellular pH range of 6.0-7.5 maintain their cytosolic pH (pH(i)) within the range 6.7-7.1. Maintenance of the resting pH(i) was Na+-dependent but unaffected by amiloride (or analogs thereof). Recovery of pH, from an intracellular acidosis was also Na+-dependent, with the rate of recovery varying with the extracellular Na+ concentration in a saturable manner (K-m = 18 mm; V-max = 10 mM H+ min(-1)). The recovery of pH. from an acid load was inhibited by amiloride but unaffected by a number of its analogs. The postulated involvement of one or more Na+/H+ exchanger(s) in the regulation of pH(i) in G. intestinalis is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:34:39