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Titolo:
Role of DnaB helicase in UV-induced illegitimate recombination in Escherichia coli
Autore:
Hanada, K; Yamashita, T; Shobuike, Y; Ikeda, H;
Indirizzi:
Kitasato Inst, Ctr Basic Res, Minato Ku, Tokyo 1088642, Japan Kitasato Inst Tokyo Japan 1088642 c Res, Minato Ku, Tokyo 1088642, Japan Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Tokyo, Inst Med Sci, Tokyo 1088639, Japan
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 17, volume: 183, anno: 2001,
pagine: 4964 - 4969
SICI:
0021-9193(200109)183:17<4964:RODHIU>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIO TRANSDUCING PHAGE; DOUBLE-STRAND BREAKS; STALLED REPLICATION FORKS; MECHANISM; DELETIONS; MUTANTS; MUTATION; PROTEIN; HOTSPOT; REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, H Kitasato Inst, Ctr Basic Res, Minato Ku, Shirokane 5-9-1, Tokyo 1088642, Japan Kitasato Inst Shirokane 5-9-1 Tokyo Japan 1088642 1088642, Japan
Citazione:
K. Hanada et al., "Role of DnaB helicase in UV-induced illegitimate recombination in Escherichia coli", J BACT, 183(17), 2001, pp. 4964-4969

Abstract

To study the involvement of DNA replication in UV-induced illegitimate recombination, we examined the effect of temperature-sensitive dnaB mutations on illegitimate recombination and found that the frequency of illegitimate recombination was reduced by an elongation-deficient mutation, dnaB14, but not by an initiation-deficient mutation, dnaB252. This result indicates that DNA replication is required for UV-induced illegitimate recombination. Inaddition, the dnaB14 mutation also affected spontaneous or UV-induced illegitimate recombination enhanced by the recQ mutation. Nucleotide sequence analyses of the recombination junctions showed that DnaB-mediated illegitimate recombination is short homology dependent. Previously, Michel et al. (B. Michel, S. Ehrlich, and M. Uzest, EMBO J. 16:430-438, 1997) showed that thermal treatment of the temperature-sensitive dnaB8 mutant induces double-stranded breaks, implying that induction of illegitimate recombination occurs. To explain the discrepancy between the observations, we propose a model for DnaB function, in which the dnaB mutations may exhibit two types of responses, early and late responses, for double-stranded break formation. In the early response, replication forks stall at damaged DNA, resulting in the formation of double-stranded breaks, and the dnaB14 mutation reduces the double-stranded breaks shortly after temperature shift-up. On the other hand,in the late response, the arrested replication forks mediated by the dnaB8mutation may induce double-stranded breaks after prolonged incubation.

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Documento generato il 29/03/20 alle ore 12:14:25