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Titolo:
Evaluation of cryoinjury of spermatozoa after slow (programmed biological freezer) or rapid (liquid nitrogen vapour) freeze-thawing techniques
Autore:
Hammadeh, ME; Szarvasy, D; Zeginiadou, T; Rosenbaum, P; Georg, T; Schmidt, W;
Indirizzi:
Univ Saarland, Coll Med, Dept Obstet & Gynaecol, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 Gynaecol, D-66421 Homburg, Germany
Titolo Testata:
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
fascicolo: 7, volume: 18, anno: 2001,
pagine: 364 - 370
SICI:
1058-0468(200107)18:7<364:EOCOSA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOOSMOTIC SWELLING TEST; HUMAN-SPERM; ULTRASTRUCTURAL-CHANGES; FUNCTIONAL INTEGRITY; CRYOPRESERVED SPERM; CHROMATIN STABILITY; MEMBRANE INTEGRITY; RAM SPERMATOZOA; SOMATIC-CELLS; MOTILITY;
Keywords:
computerized biological freezer; freeze-thawing procedure; spermatozoa chromatin and morphology; static liquid nitrogen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Hammadeh, ME Univ Saarland, Coll Med, Dept Obstet & Gynaecol, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 6421 Homburg, Germany
Citazione:
M.E. Hammadeh et al., "Evaluation of cryoinjury of spermatozoa after slow (programmed biological freezer) or rapid (liquid nitrogen vapour) freeze-thawing techniques", J AS REPROD, 18(7), 2001, pp. 364-370

Abstract

Purpose: This study was initiated to determine the negative effect (cryodamage) on human spermatozoa after freeze-thawing and to find out whether freezing of spermatozoa with a computerized biological freezer is more advantageous than freezing above static liquid nitrogen vapour with regard to spermatozoa vitality, chromatin normality, morphology, and membrane integrity. Methods: Forty-four semen samples were obtained front patients attending andrology laboratory, and each sample was divided into two aliquots. One aliquot was frozen using static liquid nitrogen vapour (G.II) and the second with a computerized biological freezer (G.III). Acridine orange was used for assessment of chromatin cryoinjury, whereas the morphology was evaluated according to WHO criteria. Hypoosmotic swelling test was used to identify rnembrane integrity and eosin-nigrosin staining was used to determine the vitality, of spermatozoa. Results: The rnean percentage of normally condensed chromatin in the native semen sample (G.I) decreased significantly, (p<.001)after freeze-thawing by using either liquid nitrogen vapour (G.II), or a biological freezer (G.III), which was significantly higher (p <.001) after freezing with liquid nitrogen vapour than after freezing with the biologicalprogrammed freezer. Morphologically normal spermatozoa decreased significantly (p <.001) in both freezing methods in comparison to the native semen samples. In addition, membrane integrity, of spermatozoa (HOS-test positive)was significantly, lower (p<.001) after the freeze-thawing procedure in G.II and 6.III compared to G.I. In both these parameters the deterioration was similar among the two freezing procedures. Finally, the mean percentage of live spermatozoa decreased significantly (p<.001) in both freezing techniques in relation to the mean value in the neat semen samples. Conclusions: Freeze-thawing procedure has a detrimental effect on chromatin, morphology,membrane integrity, and vitality of human spermatozoa not only by, freezing above static liquid nitrogen vapour but even by using a computerized biological freezer: Howe rev; the chromatin deterioration rates are significantly, higher by freezing above static liquid nitrogen vapour in comparison tofreezing with a programmed biological freezer: Therefore, we recommend theuse of this technique for freeing semen especially, when ICSI technique isconsidered as the main therapeutic procedure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:36:26