Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A sex-related difference in the hypertrophic versus hyperplastic response of vascular smooth muscle cells to repeated passaging in culture
Autore:
Bacakova, L; Pellicciari, C; Bottone, MG; Lisa, V; Mares, V;
Indirizzi:
Acad Sci Czech Republ, Inst Physiol, CZ-14220 Prague 4, Czech Republic Acad Sci Czech Republ Prague Czech Republic 4 0 Prague 4, Czech Republic Univ Pavia, Dept Biol Anim, Cell Biol Lab, I-27100 Pavia, Italy Univ Pavia Pavia Italy I-27100 Anim, Cell Biol Lab, I-27100 Pavia, Italy
Titolo Testata:
HISTOLOGY AND HISTOPATHOLOGY
fascicolo: 3, volume: 16, anno: 2001,
pagine: 675 - 684
SICI:
0213-3911(200107)16:3<675:ASDITH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUSLY HYPERTENSIVE RATS; GENDER-RELATED DIFFERENCES; DIFFERENT AGE-GROUPS; WISTAR-KYOTO RATS; ANGIOTENSIN-II; GROWTH-FACTOR; FEMALE RATS; PROLIFERATION; BETA; POLYPLOIDY;
Keywords:
rat aortic smooth muscle cells; polyploidization; gender differences; cell proliferation; flow cytometry; alpha-v integrins; vinculin; alpha-actin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Bacakova, L Acad Sci Czech Republ, Inst Physiol, Videnska 1083, CZ-14220 Prague 4, Czech Republic Acad Sci Czech Republ Videnska 1083 Prague Czech Republic 4 c
Citazione:
L. Bacakova et al., "A sex-related difference in the hypertrophic versus hyperplastic response of vascular smooth muscle cells to repeated passaging in culture", HIST HISTOP, 16(3), 2001, pp. 675-684

Abstract

Activation of growth of vascular smooth muscle cells (VSMC) in adults participates in pathogenesis of dysplastic diseases of the vascular system. In this study, we examined the impact of gender of rat donors on the degree ofhyperplastic and hypertrophic responses of VSMC in cultures subjected to repeated passaging. The cells were derived from the outgrowth zone of explants of the thoracic aorta and were studied up to passage 45. Under these conditions, the cells undergo repeated growth stimulation by the serum growth factors mimicking some pathological situations in vivo. At lower passages (5-7), the cells from both sex donors did not differ significantly in their doubling time, maximum population density, protein content and ploidy. At higher passages (40-45), we found that the hyperplastic response, monitored by doubling time and BrdU-revealed DNA synthesis, was more intense in VSMC of male origin. In contrast, female-derived cells reacted by more prominenthypertrophic changes. The latter included a relatively higher increase in the volume and protein content of cells. As indicated by the DNA content histograms and chromosome numbers, these cells also showed a higher degree ofpassage-dependent polyploidization. In addition, the female-derived VSMC were found to be more effective in adhesion to the growth support evidenced by wider spreading and higher resistance of these cells to trypsin-mediateddetachment as well as higher expression of some integrin and cytoskeletal molecules. These features could partly account for the slower proliferationand polyploidization of these cells. The results suggest that rat VSMC populations of male and female origin contain cells which are intrinsically different with respect to their capability of reacting to growth stimuli. Thelower responsiveness of female-derived cells to growth stimuli may contribute to less frequent formation of hyperplastic vascular lesions in female organisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:31:42