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Titolo:
Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diagnosis of liver cystic echinococcosis
Autore:
Paul, M; Stefaniak, J;
Indirizzi:
Karol Marcinkowski Univ Med Sci, Inst Microbiol & Infect Dis, Clin Parasit& Trop Dis, PL-60355 Poznan, Poland Karol Marcinkowski Univ Med Sci Poznan Poland PL-60355 55 Poznan, Poland
Titolo Testata:
HEPATOLOGY RESEARCH
fascicolo: 1, volume: 21, anno: 2001,
pagine: 14 - 26
SICI:
1386-6346(200109)21:1<14:COTDIA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRANULOSUS; ANTIGEN; FLUID;
Keywords:
cystic echinococcosis; dot immunobinding assay; ELISA; serodiagnosis; sterile echinococcal cysts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Paul, M Karol Marcinkowski Univ Med Sci, Inst Microbiol & Infect Dis, ClinParasit& Trop Dis, Przybyszewskiego 49, PL-60355 Poznan, Poland Karol Marcinkowski Univ Med Sci Przybyszewskiego 49 Poznan Poland PL-60355
Citazione:
M. Paul e J. Stefaniak, "Comparison of the dot immunobinding assay and two enzyme-linked immunosorbent assay kits for the diagnosis of liver cystic echinococcosis", HEPATOL RES, 21(1), 2001, pp. 14-26

Abstract

The dot immunobinding assay for the detection of hydatid antigen-specific antibodies (HA-DIA) was evaluated in patients with liver cystic and alveolar echinococcosis in comparison to two commercial ELISA kits. In 30 patients, E. granulosus infection (CE) was confirmed by histopathology or by the presence of parasite protoscoleces and/or hooks or specific antigen 5 (Ag5) in cyst fluid samples obtained by the fine needle aspiration biopsy (FNAB). Infection of E. multilocularis (AE) was diagnosed in two patients by the detection of specific anti-Em2(plus) ELISA and -Em18 Western blot antibodies and finally confirmed by histopathology. The HA-DIA using bovine hydatid antigens showed a high sensitivity in serum samples from CE patients; specific antibodies were found in 29 of 30 CE patients (96.7%). One negative result has been observed in a patient 2.6 years after radical surgery with a subsequent albendazole chemotherapy. The Echinococcosis ELISA(R) (Dialab Diagnostic) was positive in 23 CE cases (76.7%). The correlation between the HA-DIA and the Echinococcosis ELISA(R) was statistically significant. By contrast, Echinococcus granulosus IgG ELISA(R) (Bordier Affinity Products) gave positive results in only 12 of 30 CE patients (40.0%). Sera from two AE patients were high positive in all three methods analysed in our study. In non-endemic areas, due to the between-strains variations and differences in cyst immunogenic activity. related to the natural history of the parasite, a choice of an optimal method for a diagnosis of liver cystic echinococcosis has been discussed. The high diagnostic sensitivity and a faster one-step procedure, in comparison to traditional enzyme immunoassays, make the HA-DIA a very useful method for the diagnosis of CE in non-endemic areas, especially in a case of small or degenerating lesions and sterile echinococcal cysts with a low immunogenicity. The positive serology for CE frequently requires additional differentiation with E. multilocularis-specific antibodies. (C) 2001 Elsevier Science B.V, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 04:04:41