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Titolo:
Diversity of sialic acids revealed using gas chromatography/mass spectrometry of heptafluorobutyrate derivatives
Autore:
Zanetta, JP; Pons, A; Iwersen, M; Mariller, C; Leroy, Y; Timmerman, P; Schauer, R;
Indirizzi:
CNRS UMR 8576, Chim Biol Lab, F-59655 Villeneuve Dascq, France CNRS UMR 8576 Villeneuve Dascq France F-59655 5 Villeneuve Dascq, France Univ Kiel, Inst Biochem, D-24098 Kiel, Germany Univ Kiel Kiel Germany D-24098 Kiel, Inst Biochem, D-24098 Kiel, Germany
Titolo Testata:
GLYCOBIOLOGY
fascicolo: 8, volume: 11, anno: 2001,
pagine: 663 - 676
SICI:
0959-6658(200108)11:8<663:DOSARU>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
IONIZATION MASS-SPECTROMETRY; LINKED GLYCAN CHAINS; O-METHYL-GLYCOSIDES; LIQUID-CHROMATOGRAPHY; NEURAMINIC ACID; OLIGOSACCHARIDES; MONOSACCHARIDES; IDENTIFICATION; HEMAGGLUTININ; GLYCOPROTEINS;
Keywords:
Kdn; lactone; lactyl; methyl; sulfate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Zanetta, JP CNRS UMR 8576, Chim Biol Lab, F-59655 Villeneuve Dascq, FranceCNRS UMR 8576 Villeneuve Dascq France F-59655 Dascq, France
Citazione:
J.P. Zanetta et al., "Diversity of sialic acids revealed using gas chromatography/mass spectrometry of heptafluorobutyrate derivatives", GLYCOBIOLOG, 11(8), 2001, pp. 663-676

Abstract

The fine structural motifs of sialic acids, a frequent terminal monosaccharide of glycans, seem to contain essential biological properties. To identify such subtle structural differences, a reliable method was developed for the qualitative and quantitative identification of sialic acids present in different tissues and fluids. This method involved, after liberation of sialic acids by mild acid hydrolysis, their methyl esterification using diazomethane in the presence of methanol and the formation of volatile derivatives using heptafluorobutyric anhydride. The derivatives were analyzed by gas chromatography coupled to mass spectrometry in the electron impact mode. This technique allowed the separation and identification of a large variety of sialic acids, including different O-acylated forms of N-acetyl and N-glycolyl neuraminic acids and of 3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn). This method allowed also identifying 8-O-methylated and 8-O-sulfated derivatives, de-N-acetylated neuraminic acid, and 1,7-sialic acid lactones. Compounds present in very complex mixtures could be identified through their fragmentation patterns. Because of the stability of the heptafluorobutyrate derivatives, this method presents important improvements compared to the previous techniques, because it can be frequently applied on very small amounts of crude samples. This methodology will support progress in the field of the biology of sialic acids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:18:26