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Titolo:
Molecular cloning of a human Vent-like homeobox gene
Autore:
Moretti, PAB; Davidson, AJ; Baker, E; Lilley, B; Zon, LI; DAndrea, RJ;
Indirizzi:
Univ Adelaide, Dept Med, Adelaide, SA 5000, Australia Univ Adelaide Adelaide SA Australia 5000 ed, Adelaide, SA 5000, Australia Inst Med & Vet Sci, Div Human Immunol, Adelaide, SA 5000, Australia Inst Med & Vet Sci Adelaide SA Australia 5000 delaide, SA 5000, Australia Inst Med & Vet Sci, Hanson Ctr Canc Res, Adelaide, SA 5000, Australia InstMed & Vet Sci Adelaide SA Australia 5000 delaide, SA 5000, Australia Harvard Univ, Childrens Hosp, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 rd Hughes Med Inst, Boston, MA 02115 USA Womens & Childrens Hosp, Ctr Med Genet, Dept Cytogenet & Mol Genet, Adelaide, SA 5006, Australia Womens & Childrens Hosp Adelaide SA Australia 5006 de, SA 5006, Australia
Titolo Testata:
GENOMICS
fascicolo: 1-3, volume: 76, anno: 2001,
pagine: 21 - 29
SICI:
0888-7543(200108)76:1-3<21:MCOAHV>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE MORPHOGENETIC PROTEINS; DNA-BINDING SPECIFICITY; XENOPUS EMBRYOS; TRANSCRIPTIONAL REGULATION; SIGNALING PATHWAY; STEM-CELLS; HOMEODOMAIN; XVENT-2; BMP-4; EXPRESSION;
Keywords:
homeobox; homeodomain; ventralization; VENTX2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: D'Andrea, RJ Univ Adelaide, Dept Med, Adelaide, SA 5000, Australia Univ Adelaide Adelaide SA Australia 5000 SA 5000, Australia
Citazione:
P.A.B. Moretti et al., "Molecular cloning of a human Vent-like homeobox gene", GENOMICS, 76(1-3), 2001, pp. 21-29

Abstract

We have isolated a previously unknown human homeobox-containing cDNA, VENT-like homeobox-2 (VENTX2), using PCR with a bone marrow cDNA library and primers designed from the VENTX1 (alias HPX42) homeobox sequence. Here we describe the molecular cloning, chromosomal localization to 10q26.3, and functional analysis of this gene. The 2.4-kb human VENTX2 cDNA encoded a proteinwith a predicted molecular weight of 28 kDa containing a homeodomain with 65% identity to the Xenopus laevis ventralizing gene Xvent2B. VENTX2 antisera detected a 28-kDa protein in cells transfected with a VENTX2 expression construct, in a human erythroleukemic cell line and in bone marrow samples obtained from patients in recovery phase after chemotherapy. The similarityof the homeodomains from VENTX2 and the X. laevis Vent gene family places them in the same homeodomain class. Consistent with this structural classification, overexpression of VENTX2 in zebrafish embryos led to anterior truncations and failure to form a notochord, which are characteristics of ventralization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:13:28