Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE EFFECT OF PROMOTER STRENGTH IN ADENOVIRAL VECTORS CONTAINING HERPES-SIMPLEX VIRUS THYMIDINE KINASE ON CANCER GENE-THERAPY IN-VITRO AND IN-VIVO
Autore:
ELSHAMI AA; COOK JW; AMIN KM; CHOI H; PARK JY; COONROD L; SUN J; MOLNARKIMBER K; WILSON JM; KAISER LR; ALBELDA SM;
Indirizzi:
HOSP UNIV PENN,THORAC ONCOL RES LAB,3400 SPRUCE ST,809 MALONEY BLDG PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT MED,DIV PULM CRIT CARE PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT SURG,THORAC SURG SECT PHILADELPHIA PA 19104 UNIV PENN,MED CTR,INST HUMAN GENE THERAPY PHILADELPHIA PA 19104
Titolo Testata:
Cancer gene therapy
fascicolo: 4, volume: 4, anno: 1997,
pagine: 213 - 221
SICI:
0929-1903(1997)4:4<213:TEOPSI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MALIGNANT MESOTHELIOMA; IN-VIVO; BRAIN-TUMORS; EXPERIMENTAL GLIOMAS; MEDIATED TRANSFER; CELLS; MODEL; RATS; CYTOTOXICITY; REGRESSION;
Keywords:
GENE THERAPY; CANCER; ADENOVIRUS; HSV THYMIDINE KINASE; GANCICLOVIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
A.A. Elshami et al., "THE EFFECT OF PROMOTER STRENGTH IN ADENOVIRAL VECTORS CONTAINING HERPES-SIMPLEX VIRUS THYMIDINE KINASE ON CANCER GENE-THERAPY IN-VITRO AND IN-VIVO", Cancer gene therapy, 4(4), 1997, pp. 213-221

Abstract

The use of adenoviral vectors to deliver the herpes simplex virus thymidine kinase (HSVtk) gene followed by treatment with the prodrug ganciclovir (GCV) has promise for a variety of applications where excess cell proliferation is detrimental such as treatment of tumors and vascular restenosis. Optimizing this system is thus an important goal. The purpose of this study was to determine if the induction of higher levels of HSVtk expression would augment the sensitivity to GCV. This was accomplished by generating adenoviral vectors that expressed HSVtk from promoters of different efficiencies (the CMV versus RSV promoters). Despite higher levels of HSVtk expression per cell with the CMV promoter, there was no significant enhancement of antitumor effects between RSV- and CMV-driven adenovirus vectors in in vitro and in vivo studiesindicating that simply increasing HSVtk enzyme levels per cell above a minimal threshold level will not be effective in augmenting the HSVtk/GCV system. These results suggest that other strategies, e.g., the use of higher doses of GCV, augmentation of the ''bystander effect,'' the generation of mutant HSVtk genes with higher substrate affinities, the discovery of improved vectors with increased transduction efficiencies, or the development of new prodrugs with higher affinities for HSVtk will therefore be needed to enhance therapeutic responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:18:01